sepharose and 3-4-dihydroxyphenylglycol

The endoplasmic reticulum (ER) Ca2+ store plays a key role in integration and conveyance of Ca2+ signals in highly polarized neurons. The interconnected ER network in neurons generates Ca2+ signals in local domains, but the regional interaction is unclear. Here, we show that continuous or repetitive applications of caffeine produced robust Ca2+ release from the ER Ca2+ store in dendritic areas without severe store depletion, but that similar stimuli applied to soma caused rapid store depletion in acutely isolated midbrain dopamine neurons. Partial emptying of the ER Ca2+ store within a dendrite caused a similar level of store depletion in unstimulated dendrites, as well as in soma. Photobleaching and local stimulation experiments revealed that Ca2+ and the dye trapped within the ER diffused rapidly from the soma to dendrites up to 90 microm, which we could resolve, suggesting that the ER network acts as a functional tunnel for rapid Ca2+ transport. These data imply that the ER in soma acts as a Ca2+ reservoir supplying Ca2+ to the dendritic store, and that the dendritic store, hence, is able to respond to Ca2+-mobilizing input signals endurably.

Topics: Animals; Animals, Newborn; Caffeine; Calcium; Calcium Signaling; Central Nervous System Stimulants; Dendrites; Dose-Response Relationship, Drug; Dose-Response Relationship, Radiation; Electric Stimulation; Endoplasmic Reticulum; Fluorescent Dyes; In Vitro Techniques; Methoxyhydroxyphenylglycol; Neurons; Photobleaching; Rats; Rats, Sprague-Dawley; Sepharose; Substantia Nigra