sepharose and 2-2--azino-di-(3-ethylbenzothiazoline)-6-sulfonic-acid

Topics: Aerobiosis; Anti-Infective Agents; Antioxidants; Benzothiazoles; Biphenyl Compounds; Escherichia coli; Microbial Sensitivity Tests; Oxidation-Reduction; Picrates; Porphyra; Proton Magnetic Resonance Spectroscopy; Sepharose; Spectrophotometry, Ultraviolet; Spectroscopy, Fourier Transform Infrared; Staphylococcus aureus; Sulfonic Acids; Tyrosine

Water-soluble polysaccharides from Prunella vulgaris Linn (P. vulgaris) were fractionated using DEAE-Sepharose fast-flow column to obtain several eluents of water (PV-P1), 0.1M NaCl (PV-P2) and 0.2M NaCl (PV-P3). Structural analyses showed that PV-P1 had a higher molecular weight and degree of branching as compared to PV-P2 and PV-P3. Tertiary structure analyses indicated that PV-P1, PV-P2 and PV-P3 did not have triple-helical conformation. PV-P2 and PV-P3 showed stronger antioxidant activities than PV-P1, as measured radical scavenging capacities. PV-P1 showed stronger immunomodulatory activities than PV-P2 and PV-P3 in term of stimulation of the production of pro-inflammatory cytokines, including nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in murine macrophage RAW 264.7 cells. PV-P1, PV-P2 and PV-P3 did not exhibit cytotoxicities against RAW 264.7 at the concentrations tested. These results suggest that P. vulgaris polysaccharides could be explored as potential antioxidant and immunomodulatory agents for the complementary medicine or functional foods.

Topics: Animals; Antioxidants; Benzothiazoles; Biphenyl Compounds; Carbohydrate Conformation; Cell Death; Free Radical Scavengers; Glycosides; Immunologic Factors; Mice; Molecular Weight; Monosaccharides; Nitric Oxide; Picrates; Polysaccharides; Prunella; RAW 264.7 Cells; Sepharose; Spectrophotometry, Infrared; Sulfonic Acids; Uronic Acids