sdz-psc-833 and magnolol

A novel surface plasmon resonance-based P-gp ligand screening system (SPR-PLSS) combined with lentiviral particle (LVP) stabilization strategy was constructed to screen out potential P-gp inhibitors from natural products. Firstly, we constructed LVPs with high and low expression levels of P-gp. The LVPs can ensure the natural conformation of P-gp based on the principle that LVPs germinated from packaging cells will contain cell membrane fragments and P-gp they carry. Then the LVPs with high P-gp expression for active channel and LVPs with low P-gp expression for reference channel were immobilized on CM5 chip respectively. The affinity detection was thus carried out with the signal reduction on the two channels. The P-gp inhibitors, Valspodar (Val) and cyclosporin (CsA), as positive compounds, were detected to characterize the chip's activity, and the K

Topics: Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Biological Products; Biosensing Techniques; Biphenyl Compounds; Cell Line, Tumor; Cell Survival; Chemistry, Pharmaceutical; Cyclosporine; Cyclosporins; Dogs; Drug Evaluation, Preclinical; HEK293 Cells; Humans; In Vitro Techniques; Kinetics; Lentivirus; Ligands; Lignans; Madin Darby Canine Kidney Cells; MCF-7 Cells; Membrane Proteins; Resveratrol; Surface Plasmon Resonance