sch-23390 and 4-chlorophenylalanine-methyl-ester

In our previous study, the aqueous extract of Channa striatus (family: Channidae) fillet (AECSF) showed an antidepressant-like effect in mice. However, the mechanism of the antidepressant-like effect is unknown.. The objective of this study was to explore the involvement of monoamines in the antidepressant-like effect of AECSF in mice.. AECSF was prepared by steaming the fillets of C. striatus. The male ICR mice were pretreated with various monoaminergic antagonists viz., p-chlorophenylalanine (100 mg/kg, i.p.), prazosin (1 mg/kg, i.p.) and yohimbine (1 mg/kg, i.p.), SCH23390 (0.05 mg/kg, s.c.) and sulpiride (50 mg/kg, i.p.) followed by treatment with AECSF and tested in tail suspension test (TST). Two-way ANOVA with Tukey test were used at p < 0.05 for significance.. The pretreatments with p-chlorophenylalanine, prazosin and yohimbine, but not with SCH23390 and sulpiride, were able to reverse the antidepressant-like effect of AECSF in TST.. The antidepressant-like effect of AECSF may be mediated through the serotonergic and noradrenergic systems and not through the dopaminergic system.

Topics: Adrenergic Agonists; Adrenergic alpha-1 Receptor Antagonists; Adrenergic alpha-2 Receptor Antagonists; Animals; Antidepressive Agents; Benzazepines; Complex Mixtures; Depression; Dopamine D2 Receptor Antagonists; Fenclonine; Fishes; Hindlimb Suspension; Male; Mice, Inbred ICR; Prazosin; Serotonin; Serotonin Antagonists; Serotonin Receptor Agonists; Yohimbine