salicylates and pinostrobin

A valid and reliable method was established to separate six compounds from pigeon pea leaves via elution-extrusion counter-current chromatography. A solvent system composed of n-hexane/methanol/formic acid aqueous solution with pH = 3 (10:6:4, v/v) was screened to achieve satisfactory isolation from the ethanol extract of pigeon pea leaves. Four compounds, 9.2 mg of compound 1 (96.8%), 3.2 mg of 2 (88.0%), 6.2 mg of 4 (94.2%) and 25.2 mg of 5 (94.2%), were obtained by conventional elution from 100 mg of the precipitation fraction, respectively. Two compounds, 14.4 mg of 3 (96.3%) and 28.1 mg of 6 (96.6%), with high K values were obtained by the subsequent extrusion procedure. The compounds 1-6 were identified as 3-methoxy-5-(2-phenylethenyl)-phenol, pinostrobin chalcone, pinostrobin, 2-hydroxy-4-methoxy-6-(2-phenylvinyl)-benzoic acid, longistylin C and cajaninstilbene acid by quadrupole time-of-flight mass spectrometry, and

Topics: Antineoplastic Agents, Phytogenic; Benzoic Acid; Cell Proliferation; Countercurrent Distribution; Drug Screening Assays, Antitumor; Flavanones; Hep G2 Cells; Humans; Molecular Structure; Phenols; Pisum sativum; Plant Leaves; Salicylates; Stilbenes