salicylates and gamma-sitosterol

Using cholesterol, stigmasterol and sitosterol as starting materials, a series of 7-subsitituted-ster-3-yl 2-methoxybenzoate analogs were prepared through reacting with 2-methoxybenzoyl chloride and introducing some function groups, such as carbonyl, hydroxyl and various thiosemicarbazones, at 7-position of steroidal nucleus. The structures of these new compounds were characterized by IR, NMR and HRMS. Their antiproliferative activities were evaluated by using several types of cancer cells. Interestingly, the compounds displayed potent antiproliferative activity against CNE-2 (nasopharyngeal carcinoma cell lines), BEL-7402 (human liver cancer cell lines) and HepG2 (human liver cancer cell lines), suggesting that they have potential to be drug candidates for cancer treatment.

Topics: Antineoplastic Agents; Cell Line, Tumor; Cell Proliferation; Cholesterol; Drug Screening Assays, Antitumor; Hep G2 Cells; Humans; Hydroxybenzoate Ethers; Magnetic Resonance Spectroscopy; Salicylates; Sitosterols; Steroids; Stigmasterol; Structure-Activity Relationship; Thiosemicarbazones

To study the chemical constituents in ethyl acetate fraction from the Euphorbia fischeriana.. The compounds were isolated by silica gel column chromatography and HPLC, and their structures were elucidated by means of spectral analyses.. Fourteen compounds were identified as 2,4-dihydroxy-6-methoxy-3-methylacetophenone (1), 12-deoxyphorbol-13-hexadecanoate (2), ethylgallate (3), esculetin (4), 2,4-dihydroxy-6-methoxy-acetophenone (5), 12-deoxyphorbol-13-acetate (6), 12-deoxyphorbol-13,20-diacetete (7), jolkinolide B (8), 17-hydroxyjolkinolide B (9), 17-hydroxy-jolkinolide A (10), langduin C (11), 3-methylp-hydroxybenzoic acid (12), methyl gallate (13), 3, 3'-diacetyl-pyridine-4,4'-dimethoxy-2, 2', 6, 6'-tetrahydroxydiphenylmethane (14).. Compounds 3, 5, 12, 13 are isolated from this plant for the first time.

Topics: Acetophenones; Chromatography, Thin Layer; Euphorbia; Gallic Acid; Nitrates; Plant Roots; Plants, Medicinal; Salicylates; Sitosterols

Topics: Antineoplastic Agents, Phytogenic; Drugs, Chinese Herbal; Palmitates; Salicylates; Sitosterols