salicylates and ferric-nitrate

A bichromatic method is described for the determination of salicylate using a micro centrifugal analyzer. Salicylate was quantitated based on its reaction with ferric ion to produce a violet-colored complex. The method provides sample blanking and allows for rapid determination of salicylate levels in serum.

Topics: Bilirubin; Centrifugation; Ferric Compounds; Humans; Nitrates; Salicylates; Salicylic Acid

We describe a new automated method for measuring serum salicylate in the Multistat III microcentrifugal analyzer. Ferric nitrate reagent and serum blanking are used. We compare this new method, the automated Du Pont aca method, and the manual Trinder method with a "high-performance" liquid-chromatographic method. The unblanked Trinder method had the poorest correlation (r = 0.980, Sy X x = 19.1) with the chromatographic method. The serum-blanked aca and Multistat III methods showed better correlation (r = 0.995, Sy X x = 9.5 mg/L, and r = 0.991, Sy X x = 13.0 mg/L, respectively) with the chromatographic method. However, we conclude that all three colorimetric methods give clinically useful results and that the increased time, expense, and expertise required for chromatographic salicylate analysis are difficult to justify in a routine clinical laboratory.

Topics: Autoanalysis; Chromatography, High Pressure Liquid; Colorimetry; Ferric Compounds; Humans; Nitrates; Salicylates; Spectrophotometry; Ultracentrifugation

Topics: Ferric Compounds; Humans; Nitrates; Salicylates; Spectrophotometry