salicylates has been researched along with 3-toluic-acid* in 2 studies
2 other study(ies) available for salicylates and 3-toluic-acid
| Article | Year |
|---|---|
Degradation of phenol and m-toluate in Pseudomonas sp. strain EST1001 and its Pseudomonas putida transconjugants is determined by a multiplasmid system.
The utilization of phenol, m-toluate, and salicylate (Phe+, mTol+, and Sal+ characters, respectively) in Pseudomonas sp. strain EST1001 is determined by the coordinated expression of genes placed in different plasmids, i.e., by a multiplasmid system. The natural multiplasmid strain EST1001 is phenotypically unstable. In its Phe-, mTol-, and Sal- segregants, the plasmid DNA underwent structural rearrangements without a marked loss of plasmid DNA, and the majority of segregants gave revertants. The genes specifying the degradation of phenol and m-toluate were transferable to P. putida PaW340, and in this strain a new multiplasmid system with definite structural changes was formed. The 17-kilobase transposable element, a part of the TOL plasmid pWWO present in the chromosome of PaW340, was inserted into the plasmid DNA in transconjugants. In addition, transconjugant EST1020 shared pWWO-like structures. Enzyme assays demonstrated that ortho-fission reactions were used by bacteria that grew on phenol, whereas m-toluate was catabolized by a meta-fission reaction. Salicylate was a functional inducer of the enzymes of both pathways. The expression of silent metabolic pathways of phenol or m-toluate degradation has been observed in EST1001 Phe- mTol+ and Phe+ mTol- transconjugants. The switchover of phenol degradation from the ortho- to the meta-pathway in EST1033 also showed the flexibility of genetic material in EST1001 transconjugants. Topics: Benzoates; Conjugation, Genetic; DNA, Bacterial; Genes; Genes, Bacterial; Oxygen Consumption; Oxygenases; Phenol; Phenols; Phenotype; Plasmids; Pseudomonas; Restriction Mapping; Salicylates; Salicylic Acid; Species Specificity; Substrate Specificity | 1989 |
High-performance liquid chromatographic evaluation of salicyloyl pyridixol and systemic metabolites in biological samples.
An high-performance liquid chromatographic analytical method, which allows quantitative evaluation of both salicyloyl pyridixol and its metabolite salicylic acid, is reported. This method has demonstrated to possess the required specifications in terms of linearity, sensitivity, extraction recovery, reproducibility and specificity for pharmacokinetic investigations in both human subjects and experimental animals. The results obtained from an investigation on the rat are briefly discussed. Topics: Animals; Anti-Inflammatory Agents, Non-Steroidal; Benzoates; Chromatography, High Pressure Liquid; Pyridoxine; Rats; Salicylates | 1988 |