s-farnesylcysteine and 2-naphthol

s-farnesylcysteine has been researched along with 2-naphthol* in 1 studies

Other Studies

1 other study(ies) available for s-farnesylcysteine and 2-naphthol

ArticleYear
Quantitation of prenylcysteines by a selective cleavage reaction.
    Proceedings of the National Academy of Sciences of the United States of America, 1991, Nov-01, Volume: 88, Issue:21

    The allylic thioether bond of the prenylcysteines of prenylated proteins has been shown to be cleaved by 2-naphthol under alkaline conditions to yield substituted naphthopyrans. These products are readily resolved from interfering materials by HPLC and have a strongly absorbing chromophore. Thus, this reaction is suitable for quantitative analysis of prenyl substituents of proteins, and we have examined a number of tissues for their content of prenylcysteines. These amino acids are present in mammalian tissues at a concentration of 0.36-1.4 nmol/mg of protein, with a ratio of geranylgeranylcysteine to farnesylcysteine in the range of 4 to 10. Prenylcysteines were also found in the cytosolic fraction of two mouse tissues at about one-third the concentration of the whole organ. The level of these modified amino acids was found to be significantly less in a yeast, a fungus, a brown alga, a higher plant, and an insect. Again, geranylgeranylcysteine is predominant. Prenylcysteines were absent from Escherichia coli but present in an archaebacterium. The prenylcysteine content of mammalian tissue is about 1% of that of cholesterol and about equal to that of ubiquinones and dolichols. Calculations indicate that about 0.5% of all proteins are prenylated.

    Topics: 3T3 Cells; Animals; CHO Cells; Chromatography, High Pressure Liquid; Cricetinae; Cysteine; Diterpenes; HeLa Cells; Humans; Mevalonic Acid; Mice; Naphthols; Protein Processing, Post-Translational

1991