ryanodine and nitr-5

ryanodine has been researched along with nitr-5* in 1 studies

Other Studies

1 other study(ies) available for ryanodine and nitr-5

ArticleYear
Cardiac sarcoplasmic reticulum phosphorylation increases Ca2+ release induced by flash photolysis of nitr-5.
    Circulation research, 1995, Volume: 77, Issue:5

    Effects on Ca(2+)-induced Ca2+ release due to phosphorylation of sarcoplasmic reticulum (SR) proteins were investigated in isoproterenol-treated saponin-permeabilized trabeculae from rat ventricles. In these experiments, Ca2+ release from the SR was induced by a rapid change in concentration of free Ca2+ (ie, trigger Ca2+) achieved by flash photolysis of nitr-5, and the amount of Ca2+ released was assessed by measuring isometric tension. Ca2+ uptake by the SR was more rapid, and the amount of Ca2+ released by a given concentration of trigger Ca2+ was greater in isoproterenol-treated trabeculae compared with control trabeculae. However, under the same conditions of Ca2+ loading, the amplitudes of caffeine-elicited tension transients in control trabeculae were similar to those in isoproterenol-treated trabeculae, suggesting that the Ca2+ available for release was similar in the two cases. Control experiments showed that there were no significant differences in Ca2+ sensitivity of tension between isoproterenol-treated and control trabeculae. Also, application of alkaline phosphatase to trabeculae that had previously been treated with isoproterenol returned SR Ca2+ release to control levels. We conclude that the greater release of Ca2+ in isoproterenol-treated trabeculae in response to a given concentration of trigger Ca2+ is due to phosphorylation of SR proteins, most likely the Ca2+ release channel.

    Topics: Alkaline Phosphatase; Animals; Calcium Channels; Chelating Agents; Cyclic AMP; Egtazic Acid; Female; Heart; In Vitro Techniques; Isoproterenol; Male; Myocardial Contraction; Myocardium; Phosphorylation; Photolysis; Rats; Rats, Wistar; Ryanodine; Saponins; Sarcoplasmic Reticulum

1995