ru-66647 and trimethyloxamine

ru-66647 has been researched along with trimethyloxamine* in 1 studies

Other Studies

1 other study(ies) available for ru-66647 and trimethyloxamine

Article	Year
Osmolytes stimulate the reconstitution of functional 50S ribosomes from in vitro transcripts of Escherichia coli 23S rRNA. RNA (New York, N.Y.), 2002, Volume: 8, Issue:4 Functional Escherichia coli 50S ribosomal subunits can be reconstituted from their natural rRNA and protein components. However, when the assembly is performed with in vitro-transcribed 23S rRNA, the reconstitution efficiency is diminished by four orders of magnitude. We tested a variety of chemical chaperones (compounds that are typically used for protein folding), putative RNA chaperones (proteins) and ribosome-targeted antibiotics (small-molecule ligands) that might be reasoned to aid in folding and assembly. Addition of the osmolyte trimethylamine-oxide (TMAO) and the ketolide antibiotic telithromycin (HMR3647) to the reconstitution stimulates its efficiency up to 100-fold yielding a substantially improved system for the in vitro analysis of mutant ribosomes. Topics: Anti-Bacterial Agents; Base Sequence; Carbohydrates; Caseins; Escherichia coli; Ketolides; Lipids; Macrolides; Magnesium; Methylamines; Molecular Sequence Data; Mutation; Peptidyl Transferases; Plant Proteins, Dietary; Ribosomes; RNA, Messenger; RNA, Ribosomal, 23S; RNA, Transfer; Transcription, Genetic	2002

Article

Year

Osmolytes stimulate the reconstitution of functional 50S ribosomes from in vitro transcripts of Escherichia coli 23S rRNA.

RNA (New York, N.Y.), 2002, Volume: 8, Issue:4

Functional Escherichia coli 50S ribosomal subunits can be reconstituted from their natural rRNA and protein components. However, when the assembly is performed with in vitro-transcribed 23S rRNA, the reconstitution efficiency is diminished by four orders of magnitude. We tested a variety of chemical chaperones (compounds that are typically used for protein folding), putative RNA chaperones (proteins) and ribosome-targeted antibiotics (small-molecule ligands) that might be reasoned to aid in folding and assembly. Addition of the osmolyte trimethylamine-oxide (TMAO) and the ketolide antibiotic telithromycin (HMR3647) to the reconstitution stimulates its efficiency up to 100-fold yielding a substantially improved system for the in vitro analysis of mutant ribosomes.

Topics: Anti-Bacterial Agents; Base Sequence; Carbohydrates; Caseins; Escherichia coli; Ketolides; Lipids; Macrolides; Magnesium; Methylamines; Molecular Sequence Data; Mutation; Peptidyl Transferases; Plant Proteins, Dietary; Ribosomes; RNA, Messenger; RNA, Ribosomal, 23S; RNA, Transfer; Transcription, Genetic

2002