rottlerin and 4-bromophenacyl-bromide

rottlerin has been researched along with 4-bromophenacyl-bromide* in 1 studies

Other Studies

1 other study(ies) available for rottlerin and 4-bromophenacyl-bromide

ArticleYear
A phospholipase A₂ isolated from Lachesis muta snake venom increases the survival of retinal ganglion cells in vitro.
    Toxicon : official journal of the International Society on Toxinology, 2011, Mar-15, Volume: 57, Issue:4

    We have previously showed that a phospholipase A₂ isolated from Lachesis muta snake venom and named LM-PLA₂-I displayed particular biological activities, as hemolysis, inhibition on platelet aggregation, edema induction and myotoxicity. In the present work, we evaluated the effect of LM-PLA₂-I on the survival of axotomized rat retinal ganglion cells kept in vitro, as well as its mechanism of action. Our results clearly showed that treatment with LM-PLA₂-I increased the survival of ganglion cells (100% when compared to control cultures) and the treatment of LM-PLA₂-I with p-bromophenacyl bromide abolished this effect. This result indicates that the effect of LM-PLA₂-I on ganglion cell survival is entirely dependent on its enzymatic activity and the generation of lysophosphatidylcholine (LPC) may be a prerequisite to the observed survival. In fact, commercial LPC mimicked the effect of LM-PLA₂-I upon ganglion cell survival. To investigate the mechanism of action of LM-PLA₂-I, cultures were treated with chelerythrine chloride, BAPTA-AM, rottlerin and also with an inhibitor of c-junc kinase (JNKi). Our results showed that rottlerin and JNK inhibitor abolished the LM-PLA₂-I on ganglion cell survival. Taken together, our results showed that LM-PLA₂-I and its enzymatic product, LPC promoted survival of retinal ganglion cells through the protein kinase C pathway and strongly suggest a possible role of the PLA₂ enzyme and LPC in controlling the survival of axotomized neuronal cells.

    Topics: Acetonitriles; Acetophenones; Animals; Benzophenanthridines; Benzopyrans; Benzothiazoles; Cell Survival; Cells, Cultured; Crotalid Venoms; Egtazic Acid; Enzyme Inhibitors; Lysophosphatidylcholines; Phospholipases A2; Rats; Retinal Ganglion Cells

2011
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