rifampin and trichlorosilane

Providing a long period of sustained antibiotic release is one of the important challenges in the development of clinical shunts for long-term implantation. A cast-molding approach was used to load rifampicin into the silicone precursor before curing. Sustained in vitro release from rifampicin-loaded silicone for upwards of 110 days at a level of approximately 2-4 microg/cm2 per day was achieved. Quantitative comparisons of Staphylococcus epidermidis adhesion on untreated and rifampicin-loaded silicone surfaces were carried out to demonstrate the effect of rifampicin to discourage the bacterial adhesion. It was shown that the fresh 8.3% rifampicin-loaded silicone decreased bacterial adherence by 99.8%. Bacterial adherence on rifampicin-loaded silicone surfaces after 90 days release (eluted silicone) was decreased by 94.8%. A new approach was used to tune the initial burst effect and prolong long lasting release by introducing self-assembled perfluorodecyltrichlorosilane (FAS) and octadecyltrichlorosilane (OTS) layers. FAS layered structures can tune the burst effect and prolong the subsequent continuous release to achieve the long-term delivery. Significant decreases in initial burst effect (70.3% for multi-FAS layers and 39.7% for FAS monolayer) and enhanced long-term release (approx. 10 microg/cm2 per day for FAS monolayer for 110 days and approx. 15 microg/cm2 per day for multi-FAS layers for 60 days) were observed.

Topics: Antibiotics, Antitubercular; Bacterial Adhesion; Catheters, Indwelling; Coated Materials, Biocompatible; Delayed-Action Preparations; Hydrocephalus; Prosthesis-Related Infections; Rifampin; Silanes; Silicones; Staphylococcus epidermidis; Time Factors