rifampin and 3-toluic-acid

rifampin has been researched along with 3-toluic-acid* in 1 studies

Other Studies

1 other study(ies) available for rifampin and 3-toluic-acid

ArticleYear
Protein binding in vivo to OP2 promoter of the Pseudomonas putida TOL plasmid.
    Biochemistry and molecular biology international, 1998, Volume: 46, Issue:5

    The transcription of OP2 encoding enzymes for m-toluate catabolism on the Pseudomonas putida TOL plasmid is activated by basal-level XylS protein in the presence of m-toluate or by overproduced XylS protein in the absence of m-toluate. In this study, in vivo dimethyl sulfate (DMS) footprinting was performed to understand the mechanism of transcriptional regulation of OP2 promoter by XylS. In the presence of overproduced XylS without m-toluate, several protected nucleotides were observed, indicating the binding of RNA polymerase to DNA. However, the protection was canceled upon addition of m-toluate. These results suggest that RNA polymerase is retained by XylS on the OP2 promoter in the absence of inducer, and is released by m-toluate binding to XylS, concomitant with transcription.

    Topics: Bacterial Proteins; Base Sequence; Benzoates; DNA Footprinting; DNA Methylation; DNA-Binding Proteins; DNA-Directed RNA Polymerases; Gene Expression Regulation, Bacterial; Genes, Bacterial; Operon; Plasmids; Promoter Regions, Genetic; Protein Binding; Pseudomonas putida; Rifampin; Sulfuric Acid Esters; Trans-Activators; Transcription, Genetic

1998