ribothymidine and pentaerythrityl-triacrylate

Normal and modified urinary nucleosides represent potential biomarkers for cancer diagnosis. To selectively extract modified nucleosides, we developed a molecularly imprinted polymer (MIP) of 5-methyluridine as selective material for molecularly imprinted solid-phase extraction (MISPE). The MIPs were obtained from vinyl-phenylboronate ester derivative of the template, acrylamide and pentaerythritol triacrylate co-polymer, and were tested in batch and cartridge experiments with aqueous samples. Our results indicated that the imprinted polymer was selective for pyrimidine nucleosides with a K(d) and a B(max) of 46 microM and 18 micromol/g, respectively. Finally, a MISPE of the most common pyrimidine nucleoside cancer markers in urine sample was realized.

Topics: Acrylamide; Acrylates; Animals; Biomarkers; Chromatography, High Pressure Liquid; Dogs; Mass Spectrometry; Microscopy, Electron, Scanning; Molecular Imprinting; Neoplasms; Polymers; Propylene Glycols; Pyrimidine Nucleosides; Reproducibility of Results; Sensitivity and Specificity; Solid Phase Extraction; Uridine; Vinyl Compounds