ribothymidine and 5-10-methylenetetrahydrofolic-acid

ribothymidine has been researched along with 5-10-methylenetetrahydrofolic-acid* in 1 studies

Other Studies

1 other study(ies) available for ribothymidine and 5-10-methylenetetrahydrofolic-acid

Article	Year
Methylenetetrahydrofolate-dependent biosynthesis of ribothymidine in transfer RNA of Streptococcus faecalis. Evidence for reduction of the 1-carbon unit by FADH2. The Journal of biological chemistry, 1980, May-25, Volume: 255, Issue:10 The methyl carbon of ribothymidine in Loop IV of the tRNA of Streptococcus faecalis, Bacillus subtilis, and some other microorganisms is derived directly from 5,10-methylenetetrahydrofolate, not S-adenosylmethionine. The pure enzyme from S. faecalis also requires FADH2. We have obtained evidence that tetrahydrofolate is a product of the reaction and demonstrated that label from [5-3H]5-deazaFMNH2 is incorporated into the methyl moiety of ribothymidine. These data indicate that the enzyme uses methylenetetrahydrofolate solely as a 1-carbon donor and employs FADH2 as a reducing agent in vitro according to the following reaction: tRNA(U psi C) + CH2 = THF + FADH2 leads to tRNA(T psi C) + THF + FAD. Topics: Enterococcus faecalis; Flavin-Adenine Dinucleotide; Methylation; Methyltransferases; Oxidation-Reduction; Ribonucleosides; RNA, Transfer; Species Specificity; Tetrahydrofolates; Uridine	1980

Article

Year

Methylenetetrahydrofolate-dependent biosynthesis of ribothymidine in transfer RNA of Streptococcus faecalis. Evidence for reduction of the 1-carbon unit by FADH2.

The Journal of biological chemistry, 1980, May-25, Volume: 255, Issue:10

The methyl carbon of ribothymidine in Loop IV of the tRNA of Streptococcus faecalis, Bacillus subtilis, and some other microorganisms is derived directly from 5,10-methylenetetrahydrofolate, not S-adenosylmethionine. The pure enzyme from S. faecalis also requires FADH2. We have obtained evidence that tetrahydrofolate is a product of the reaction and demonstrated that label from [5-3H]5-deazaFMNH2 is incorporated into the methyl moiety of ribothymidine. These data indicate that the enzyme uses methylenetetrahydrofolate solely as a 1-carbon donor and employs FADH2 as a reducing agent in vitro according to the following reaction: tRNA(U psi C) + CH2 = THF + FADH2 leads to tRNA(T psi C) + THF + FAD.

Topics: Enterococcus faecalis; Flavin-Adenine Dinucleotide; Methylation; Methyltransferases; Oxidation-Reduction; Ribonucleosides; RNA, Transfer; Species Specificity; Tetrahydrofolates; Uridine

1980