rhoifolin and neoeriocitrin

To establish a method of quality assurance for mass-screening (QAMS) for simultaneously determining six flavonoids in Hongzhu Capsules.. An HPLC (high performance liquid chromatography) method was developed as QAMS to determine neoeriocitrin, prunin, naringin, rhoifolin, miliditin and naringenin in Hongzhu Capsules. Using naringin as the internal reference substance, relative correction factors (RCF) of the five flavonoids were determined and given as follows (1.05, 0.782, 1.89, 1.27, 0.532). The contents of the flavonoids in Hongzhu Capsules were determined by QAMS and validated by the external standard method.. RCF values were determined by HPLC with good reproducibility. No significant difference between the quantitative results of QAMS and external standard method was observed.. The present-developed method is convenient and accurate to determine multiple components when some standard substances are unavailable, thus considered as a potential method for quality control of Hongzhu Capsules.

Topics: Capsules; Chromatography, High Pressure Liquid; Disaccharides; Drugs, Chinese Herbal; Flavanones; Flavonoids; Glycosides; Phlorhizin; Plants, Medicinal; Quality Control; Reproducibility of Results

To investigate the impurities in naringin extracted from Citrus grandis 'Tomentosa'.. High performance liquid chromatographies coupled with photodiode array and electrospray ionization mass spectrometry detectors (HPLC-PDA/ESI-MS/MS) were applied to investigate the impurities, and their structures were elucidated by spectral data analyses. Quantification was carried out by main component self-compare with correction factor according to ICH guidelines.. Rhoifolin and neoeriocitrin were identified as major impurities. The correction factors of rhoifolin and neoeriocitrin were 1.82 and 1.02, respectively tested by HPLC method. The content of rhoifolin ranged from 0.742% to 0.926%, and the content of neoeriocitrin ranged from 0.335% to 0.464%. The gross impurities were less than 1.5%.. The categories and quantities of impurities in naringin product are relatively stable. The research provides a way of specification and verification for the analysis of impurities and objective evidence for security assessment of naringin product.

Topics: Chromatography, High Pressure Liquid; Citrus; Disaccharides; Flavanones; Flavonoids; Fruit; Glycosides; Hypolipidemic Agents; Molecular Structure; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry