rhodanine has been researched along with thiazolyl-blue* in 1 studies
1 other study(ies) available for rhodanine and thiazolyl-blue
Article | Year |
---|---|
Novel rhodanine derivatives induce growth inhibition followed by apoptosis.
We have designed and synthesized three novel compounds, 5-isopropylidiene derivatives of 3-dimethyl-2-thio-hydantoin (ITH-1), 3-ethyl-2-thio-2,4-oxazolidinedione (ITO-1), and 5-benzilidene-3-ethyl rhodanine (BTR-1), and have tested their chemotherapeutic properties. Our results showed that all three compounds induced cytotoxicity in a time- and concentration-dependent manner on leukemic cell line, CEM. Among the compounds tested, BTR-1 was 5- to 7-fold more potent than ITH-1 and ITO-1 when compared by trypan blue and MTT assays. IC(50) value of BTR-1 was estimated to be <10μM. Both cell cycle analysis and tritiated thymidine assays revealed that BTR-1 affects DNA replication by inducing a block at S phase. BTR-1 treatment led to increased level of ROS production and DNA strand breaks suggesting activation of apoptosis for induction of cell death. Topics: Antineoplastic Agents; Apoptosis; Cell Line, Tumor; Cell Membrane; Cell Proliferation; Cell Survival; Coloring Agents; DNA Fragmentation; Dose-Response Relationship, Drug; Humans; L-Lactate Dehydrogenase; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma; Reactive Oxygen Species; Rhodanine; Tetrazolium Salts; Thiazoles; Trypan Blue | 2010 |