rebaudioside-m and steviol

A silica gel orthogonal method using acetonitrile: water was developed for the analyses of fractions rich in very polar steviol glycosides and resolve regions of co-elution of these compounds in reversed-phase. Additionally, we also used this normal phase analytical method to scale up the purification process of steviol glycosides. Using these approaches, one novel minor tetra-glucopyranosyl diterpene glycosides together with three known compounds were purified from a commercial

Topics: Chromatography, High Pressure Liquid; Diterpenes, Kaurane; Glucosides; Glycosides; Magnetic Resonance Spectroscopy; Silica Gel; Stevia; Tandem Mass Spectrometry; Trisaccharides