raffinose has been researched along with 3-methyladenine* in 2 studies
2 other study(ies) available for raffinose and 3-methyladenine
| Article | Year |
|---|---|
Evidence for a dual control of macroautophagic sequestration and intracellular trafficking of N-linked glycoproteins by the trimeric G(i3) protein in HT-29 cells.
The trimeric G(i3) protein-dependent lysosomal-autophagic pathway is responsible for the degradation of a pool of N-linked glycoproteins in the human colon cancer HT-29 cell line. Here we have followed the fate of N-glycans using HT-29 cells either overexpressing the wild-type G alpha(i3) protein or transfected with different mutants of the G alpha(i3) protein. The stabilization of N-glycans was dependent upon the inhibition of autophagic sequestration by either 3-methyladenine (3-MA) or pertussis toxin (PTX). However, PTX allowed the processing of high-mannose glycans whereas 3-MA did not. The destabilization of the Golgi apparatus by brefeldin A, which interrupts the intracellular trafficking of N-linked glycoproteins along the secretory pathway, did not interfere with the macroautophagic pathway. These results suggest that the lysosomal-autophagic pathway is not dependent upon the integrity of the Golgi apparatus and points to differences between the molecular properties of two membrane flow processes (macroautophagy, exocytic pathway) controlled by the trimeric G(i3) protein. Topics: Adenine; Autophagy; Brefeldin A; Cyclopentanes; Exocytosis; Glycoproteins; Golgi Apparatus; GTP-Binding Protein alpha Subunits, Gi-Go; HT29 Cells; Humans; Lysosomes; Mutation; Pertussis Toxin; Polysaccharides; Raffinose; Transfection; Virulence Factors, Bordetella | 1997 |
Use of [3H]raffinose as a specific probe of autophagic sequestration.
The trisaccharide [3H]raffinose, introduced into the cytosol of isolated rat hepatocytes by means of electropermeabilization, was sequestered autophagically and accumulated in lysosomes and pre-lysosomal vacuoles. In contrast to the disaccharide [14C]sucrose previously used as a sequestration probe, raffinose was not taken up by the mitochondria. The sequestration of raffinose was completely inhibited by the autophagy suppressor 3-methyladenine, stressing its potential utility as a specific probe of hepatocytic autophagy. Topics: Adenine; Animals; Autophagy; Digitonin; Liver; Lysosomes; Male; Mitochondria, Liver; Oligosaccharides; Phagocytosis; Phagosomes; Raffinose; Rats; Rats, Inbred Strains; Sucrose | 1986 |