quinaldine-blue and 1-9-dimethylmethylene-blue

The polarization optical analysis of human blood platelets was carried out by means of topo-optical staining reactions. Similar studies have not been performed so far. With this approach we were able to demonstrate the spatially oriented nature of glycoprotein components in the platelet membrane. Using a sialic acid specific topo-optical reaction the sialic acid component of human platelet membrane was selectively demonstrated and the even distribution of sialic acid residues on the membrane surface was also suggested. Polarization optical analysis has shown a membrane-parallel orientation of oligosaccharide chains carrying sialic acids.

Topics: Blood Platelets; Carbocyanines; Cell Membrane; Glycoproteins; Histocytochemistry; Humans; Hydrolysis; Methylene Blue; Neuraminidase; Staining and Labeling; Tolonium Chloride