pyrromethene-546 and nile-red

pyrromethene-546 has been researched along with nile-red* in 2 studies

Other Studies

2 other study(ies) available for pyrromethene-546 and nile-red

ArticleYear
High-content imaging of neutral lipid droplets with 1,6-diphenylhexatriene.
    BioTechniques, 2011, Volume: 51, Issue:1

    Neutral lipid droplets (LDs) are dynamic lipid storage organelles found in all eukaryotic cells from yeast to mammals and higher plants. LDs are important to many physiological processes that include basic cellular maintenance, metabolism, and diverse medical pathologies. LD accumulation has been studied extensively by a range of methods, but particularly by microscopy with several fluorescent dyes extensively used for qualitative and quantitative imaging. Here, we compared established LD stains Nile Red and BODIPY 493/503 to the 4', 6-diamidino-2-phenylindole (DAPI)-range dye 1,6-diphenyl-1,3,5-hexatriene (DPH; excitation/emission λmax=350 nm/420 nm) using high-content image analysis. HeLa cells treated with oleic acid or vehicle were used to compare staining patterns between DPH and Nile Red as well as DPH and the LD protein adipophilin. DPH, Nile Red, and BODIPY 493/503 were compared as assay reagents in oleic acid dose-response experiments. Treatment of MCF-7 cells with sodium butyrate was used as a second cellular system for high-content analysis of LD formation. In this experimental context, we demonstrate the compatibility of DPH with GFP, a technical limitation of Nile Red and BODIPY 493/503 dyes. These data show that DPH has comparable sensitivity and specificity to that of Nile Red. Z'-factor analysis of dose-response experiments indicated that DPH and BODIPY 493/503 are well suited for quantitative analysis of LDs for high-throughput screening (HTS) applications.

    Topics: Boron Compounds; Cell Line, Tumor; Cytoplasmic Granules; Diphenylhexatriene; Fluorescent Dyes; HeLa Cells; Humans; Lipids; Membrane Proteins; Microscopy, Fluorescence; Oxazines; Perilipin-2; Staining and Labeling

2011
Fluorescent detection of lipid droplets and associated proteins.
    Current protocols in cell biology, 2007, Volume: Chapter 24

    Most eukaryotic cells can store excess lipid in cytosolic lipid droplets. This unit discusses techniques for the visualization of lipid droplets and associated proteins in cultured mammalian cells. Protocols for the detection of lipid droplets with nile red and BODIPY 493/503 are included. The differences in the spectral properties of these two lipophilic dyes and advantages of each are discussed. The best method for combining visualization of intracellular lipid droplets with indirect immunofluorescent detection of lipid droplet-associated proteins is described. Techniques for sample fixation and permeabilization must be chosen carefully to avoid alterations to lipid droplet morphology. Immunofluorescent detection of adipophilin, a broadly expressed, lipid droplet-associated protein, widely used as a marker for lipid droplet accumulation, is presented as an example. Finally, a simple protocol for enhancing lipid droplet accumulation through supplementation with excess fatty acid is included.

    Topics: Animals; Biomarkers; Boron Compounds; Cells, Cultured; Culture Media; Cytosol; Eukaryotic Cells; Fatty Acids; Fluorescent Antibody Technique, Indirect; Fluorescent Dyes; Indicators and Reagents; Lipids; Mammals; Membrane Proteins; Microscopy, Fluorescence; Oxazines; Peptides; Perilipin-2

2007