pyrophosphate has been researched along with inositol-4-5-bisphosphate* in 1 studies
1 other study(ies) available for pyrophosphate and inositol-4-5-bisphosphate
| Article | Year |
|---|---|
The inositol pyrophosphate synthesis pathway in Trypanosoma brucei is linked to polyphosphate synthesis in acidocalcisomes.
Inositol pyrophosphates are novel signaling molecules possessing high-energy pyrophosphate bonds and involved in a number of biological functions. Here, we report the correct identification and characterization of the kinases involved in the inositol pyrophosphate biosynthetic pathway in Trypanosoma brucei: inositol polyphosphate multikinase (TbIPMK), inositol pentakisphosphate 2-kinase (TbIP5K) and inositol hexakisphosphate kinase (TbIP6K). TbIP5K and TbIP6K were not identifiable by sequence alone and their activities were validated by enzymatic assays with the recombinant proteins or by their complementation of yeast mutants. We also analyzed T. brucei extracts for the presence of inositol phosphates using polyacrylamide gel electrophoresis and high-performance liquid chromatography. Interestingly, we could detect inositol phosphate (IP), inositol 4,5-bisphosphate (IP Topics: Animals; Biosynthetic Pathways; Carbohydrate Metabolism; Diphosphates; Inositol 1,4,5-Trisphosphate; Inositol Phosphates; Phosphotransferases (Alcohol Group Acceptor); Phosphotransferases (Phosphate Group Acceptor); Phytic Acid; Polyphosphates; Recombinant Proteins; Signal Transduction; Trypanosoma brucei brucei | 2017 |