pyrophosphate has been researched along with glucose-1-phosphate* in 2 studies
2 other study(ies) available for pyrophosphate and glucose-1-phosphate
| Article | Year |
|---|---|
Kinetic evaluation of glucose 1-phosphate analogues with a thymidylyltransferase using a continuous coupled enzyme assay.
Cps2L, a thymidylytransferase, is the first enzyme in Streptococcus pneumoniae L-rhamnose biosynthesis and an antibacterial target. We herein report the evaluation of six sugar phosphate analogues selected to further probe Cps2L substrate tolerance. A modified continuous spectrophotometric assay was employed for facile detection of pyrophosphate (PPi) released from nucleotidylyltransfase-catalysed condensation of sugar 1-phosphates and nucleoside triphosphates to produce sugar nucleotides. Additionally, experiments using waterLOGSY NMR spectroscopy were investigated as a complimentary method to evaluate binding affinity to Cps2L. Topics: Anti-Bacterial Agents; Bacterial Proteins; Diphosphates; Enzyme Assays; Enzyme Inhibitors; Glucosephosphates; Kinetics; Nucleotidyltransferases; Recombinant Proteins; Spectrophotometry; Streptococcus pneumoniae | 2015 |
Pyrophosphate may be involved in regulation of bacterial glycogen synthesis.
Inorganic pyrophosphate is a potent inhibitor of the enzyme that catalyzes synthesis of the glucosyl donor for Escherichia coli glycogen synthesis, ADP-glucose pyrophosphorylase. The Ki is determined to be 40 microM and the substrate ATP, the activator, fructose 1,6-P2 or the allosteric inhibitor, AMP do not greatly affect the inhibition. PPi exhibits mixed type inhibition with the other substrate, glucose 1-P. The potential regulation of glycogen synthesis by PPi is discussed. Topics: Adenosine Diphosphate Glucose; Carbon Radioisotopes; Diphosphates; Escherichia coli; Glucose-1-Phosphate Adenylyltransferase; Glucosephosphates; Glycogen; Kinetics; Nucleotidyltransferases | 1983 |