pyrophosphate and 8-hydroxyguanosine

pyrophosphate has been researched along with 8-hydroxyguanosine* in 1 studies

Other Studies

1 other study(ies) available for pyrophosphate and 8-hydroxyguanosine

Article	Year
A new proofreading mechanism for lesion bypass by DNA polymerase-λ. EMBO reports, 2011, Dec-23, Volume: 13, Issue:1 Replicative DNA polymerases (DNA pols) increase their fidelity by removing misincorporated nucleotides with their 3' → 5' exonuclease activity. Exonuclease activity reduces translesion synthesis (TLS) efficiency and TLS DNA pols lack 3' → 5' exonuclease activity. Here we show that physiological concentrations of pyrophosphate (PP(i)) activate the pyrophosphorolytic activity by DNA pol-λ, allowing the preferential excision of the incorrectly incorporated A opposite a 7,8-dihydro-8-oxoguanine lesion, or T opposite a 6-methyl-guanine, with respect to the correct C. This is the first example of an alternative proofreading mechanism used during TLS. Topics: Deoxyadenine Nucleotides; Diphosphates; DNA Breaks, Single-Stranded; DNA Polymerase beta; DNA Repair; DNA Replication; Enzyme Activation; Guanosine; Humans	2011

Article

Year

A new proofreading mechanism for lesion bypass by DNA polymerase-λ.

EMBO reports, 2011, Dec-23, Volume: 13, Issue:1

Replicative DNA polymerases (DNA pols) increase their fidelity by removing misincorporated nucleotides with their 3' → 5' exonuclease activity. Exonuclease activity reduces translesion synthesis (TLS) efficiency and TLS DNA pols lack 3' → 5' exonuclease activity. Here we show that physiological concentrations of pyrophosphate (PP(i)) activate the pyrophosphorolytic activity by DNA pol-λ, allowing the preferential excision of the incorrectly incorporated A opposite a 7,8-dihydro-8-oxoguanine lesion, or T opposite a 6-methyl-guanine, with respect to the correct C. This is the first example of an alternative proofreading mechanism used during TLS.

Topics: Deoxyadenine Nucleotides; Diphosphates; DNA Breaks, Single-Stranded; DNA Polymerase beta; DNA Repair; DNA Replication; Enzyme Activation; Guanosine; Humans

2011