pyrophosphate has been researched along with 3-3--5-5--tetramethylbenzidine* in 3 studies
3 other study(ies) available for pyrophosphate and 3-3--5-5--tetramethylbenzidine
| Article | Year |
|---|---|
FeMoO
Topics: Benzidines; Catalysis; Chromogenic Compounds; Colorimetry; Diphosphates; Drinking Water; Hydrogen Peroxide; Iron; Limit of Detection; Molybdenum; Nanospheres; Oxidation-Reduction; Water Pollutants, Chemical | 2022 |
Restoring the Oxidase-Like Activity of His@AuNCs for the Determination of Alkaline Phosphatase.
Topics: Alkaline Phosphatase; Benzidines; Catalysis; Colorimetry; Diphosphates; Gold; Humans; Hydrolysis; Metal Nanoparticles; Oxidation-Reduction; Oxidoreductases; Reproducibility of Results | 2021 |
Spectrophotometric determination of the activity of alkaline phosphatase and detection of its inhibitors by exploiting the pyrophosphate-accelerated oxidase-like activity of nanoceria.
The oxidase-like activity of nanoceria is low. This limits its practical applications. It is demonstrated here that pyrophosphate ion (PPi) can improve the oxidase-like activity of nanoceria. Specifically, nanoceria catalyzes the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to give a blue product (oxTMB) with an absorption peak at 645 nm in the presence of PPi. If, however, alkaline phosphatase (ALP) is present, it will hydrolyze PPi, and this results in a decreased oxidase-like activity of nanoceria. Hence, less blue oxTMB willl be formed. On the other hand, if the ALP inhibitor Na Topics: Alkaline Phosphatase; Benzidines; Catalysis; Cerium; Diphosphates; Enzyme Inhibitors; Humans; Hydrolysis; Limit of Detection; Oxidation-Reduction; Oxidoreductases; Spectrophotometry; Vanadates | 2019 |