pyrimidinones and bis(1-3-diethylthiobarbiturate)trimethineoxonol

By comparing the time-courses of rapid optical changes in the garfish olfactory nerve evoked by electric stimulation with those of mechanical changes observed at the site of optical recording, the origin of optical changes in the nerve has been investigated. Based on the finding that the time-course of the birefringence change accurately coincides with that of swelling of the nerve, optical changes are interpreted as being brought about by invasion of water into the superficial layer of the nerve fibers. A close relationship has also been demonstrated between nerve swelling and changes in light scattering and in dye absorbance.

Topics: Animals; Birefringence; Brachyura; Diffusion Chambers, Culture; Electric Stimulation; Electrophysiology; Fishes; Fluorescent Dyes; In Vitro Techniques; Light; Nerve Fibers; Olfactory Nerve; Pyrimidinones; Scattering, Radiation; Staining and Labeling; Thiobarbiturates

The orientation and morphology of the endothelium lining the cardiovascular system may result from hemodynamic forces acting on the endothelial cells. To investigate the flow effects at the membrane level, we have examined the variations of the fluorescence intensity of two membrane-sensitive dyes, merocyanine 540 and bis(1,3-diethylthiobarbiturate)trimethineoxonol, (i) as a function of flow shear stress and (ii) with the onset or cessation of the flow. We found a time-dependent decrease in fluorescence intensity with the onset of the flow with an exponential approach to steady state of the order of 1 min. The process is reversible; when the flow is stopped the fluorescence intensity returns to its original value. The polarization of the endothelial cell membranes or, more precisely, the amplitude of the fluorescence intensity responses is an increasing function of the shear stress (up to 120 dynes/cm2). Assuming the equilibrium potential for K+ is more hyperpolarized than the resting potential and using valinomycin, we have deduced from the sign of the ionophore effects that the flow hyperpolarizes the endothelial cell membrane.

Topics: Animals; Cattle; Endothelium, Vascular; Fluorescence Polarization; Hemodynamics; Kinetics; Membrane Potentials; Potassium; Pulmonary Artery; Pyrimidinones; Thiobarbiturates; Time Factors; Valinomycin