pyrachlostrobin has been researched along with fipronil* in 3 studies
3 other study(ies) available for pyrachlostrobin and fipronil
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Modification of the head proteome of nurse honeybees (Apis mellifera) exposed to field-relevant doses of pesticides.
Understanding the effect of pesticides on the survival of honeybee colonies is important because these pollinators are reportedly declining globally. In the present study, we examined the changes in the head proteome of nurse honeybees exposed to individual and combined pesticides (the fungicide pyraclostrobin and the insecticide fipronil) at field-relevant doses (850 and 2.5 ppb, respectively). The head proteomes of bees exposed to pesticides were compared with those of bees that were not exposed, and proteins with differences in expression were identified by mass spectrometry. The exposure of nurse bees to pesticides reduced the expression of four of the major royal jelly proteins (MRJP1, MRJP2, MRJP4, and MRJP5) and also several proteins associated with carbohydrate metabolism and energy synthesis, the antioxidant system, detoxification, biosynthesis, amino acid metabolism, transcription and translation, protein folding and binding, olfaction, and learning and memory. Overall, when pyraclostrobin and fipronil were combined, the changes in protein expression were exacerbated. Our results demonstrate that vital proteins and metabolic processes are impaired in nurse honeybees exposed to pesticides in doses close to those experienced by these insects in the field, increasing their susceptibility to stressors and affecting the nutrition and maintenance of both managed and natural colonies. Topics: Animals; Bees; Conservation of Natural Resources; Fatty Acids; Fungicides, Industrial; Insect Proteins; Insecticides; Pesticides; Proteome; Proteomics; Pyrazoles; Strobilurins | 2020 |
Field-relevant doses of the systemic insecticide fipronil and fungicide pyraclostrobin impair mandibular and hypopharyngeal glands in nurse honeybees (Apis mellifera).
Global decreases in bee populations emphasize the importance of assessing how environmental stressors affect colony maintenance, especially considering the extreme task specialization observed in honeybee societies. Royal jelly, a protein secretion essential to colony nutrition, is produced by nurse honeybees, and development of bee mandibular glands, which comprise a reservoir surrounded by secretory cells and hypopharyngeal glands that are shaped by acini, is directly associated with production of this secretion. Here, we examined individual and combined effects of the systemic fungicide pyraclostrobin and insecticide fipronil in field-relevant doses (850 and 2.5 ppb, respectively) on mandibular and hypopharyngeal glands in nurse honeybees. Six days of pesticide treatment decreased secretory cell height in mandibular glands. When pyraclostrobin and fipronil were combined, the reservoir volume in mandibular glands also decreased. The total number of acini in hypopharyngeal glands was not affected, but pesticide treatment reduced the number of larger acini while increasing smaller acini. These morphological impairments appeared to reduce royal jelly secretion by nurse honeybees and consequently hampered colony maintenance. Overall, pesticide exposure in doses close to those experienced by bees in the field impaired brood-food glands in nurse honeybees, a change that could negatively influence development, survival, and colony maintenance. Topics: Animals; Bees; Exocrine Glands; Fatty Acids; Fungicides, Industrial; Histocytochemistry; Insecticides; Pyrazoles; Strobilurins | 2017 |
Effect of paste processing on residue levels of imidacloprid, pyraclostrobin, azoxystrobin and fipronil in winter jujube.
The changes of imidacloprid, pyraclostrobin, azoxystrobin and fipronil residues were studied to investigate the carryover of pesticide residues in winter jujube during paste processing. A multi-residue analytical method for winter jujube was developed based on the QuEChERS approach. The recoveries for the pesticides were between 87.5% and 116.2%. LODs ranged from 0.002 to 0.1 mg kg(-1). The processing factor (Pf) is defined as the ratio of pesticide residue concentration in the paste to that in winter jujube. Pf was higher than 1 for the removal of extra water, and other steps were generally less than 1, indicating that the whole process resulted in lower pesticide residue levels in paste. Peeling would be the critical step for pesticide removal. Processing factors varied among different pesticides studied. The results are useful to address optimisation of the processing techniques in a manner that leads to considerable pesticide residue reduction. Topics: Carbamates; Food Analysis; Food Contamination; Food Handling; Imidazoles; Insecticides; Methacrylates; Molecular Structure; Neonicotinoids; Nitro Compounds; Pesticide Residues; Pyrazoles; Pyrimidines; Strobilurins; Ziziphus | 2014 |