punicalagin and ethyl-acetate

It was aimed at determining which polyphenolic compound(s) in pomegranate mesocarp extract (PME) is liable for the antioxidant, anti-glycation and anti-CD36 activities.. The PME was fractionated using liquid-liquid extraction method. The fractions were tested for their polyphenolic content, antioxidant potency, anti-glycation activity and anti-CD36 potential. The metabolite compositions of PME and derived fractions were investigated in an untargeted manner using metabolomics in relation to its antioxidant and anti-glycation activities.. The ethyl acetate and. This work demonstrated the protective effect of the non-edible part of the pomegranate fruit and showed that gallic and ellagic acids account for the antioxidant and anti-glycation activities while punicalagin is liable for the anti-CD36 activity of PME.

Topics: 1-Butanol; Antioxidants; Chromatography, Liquid; Ellagic Acid; Lythraceae; Plant Extracts; Pomegranate; Tandem Mass Spectrometry

In the course of screening for anti-dementia agents from natural products, two beta-secretase (BACE1) inhibitors were isolated from the husk of pomegranate (Punica granatum) by activity-guided purification. They were identified as ellagic acid and punicalagin with IC50 values of 3.9 x10(-6) and 4.1x10(-7) M and Ki values of 2.4x10(-5) and 5.9x10(-7) M, respectively. The compounds were non-competitive inhibitors with a substrate in the Dixon plot. Ellagic acid and punicalagin were less inhibitory to alpha-secretase (TACE) and other serine proteases such as chymotrypsin, trypsin, and elastase, thus indicating that they were relatively specific inhibitors of BACE1.

Topics: Acetates; Amyloid Precursor Protein Secretases; Aspartic Acid Endopeptidases; Butanols; Catechin; Dose-Response Relationship, Drug; Ellagic Acid; Endopeptidases; Enzyme Inhibitors; Fruit; Humans; Hydrolyzable Tannins; Lythraceae; Plant Extracts; Plants, Medicinal; Substrate Specificity