pulmicort and 4-aminophenylphosphate

pulmicort has been researched along with 4-aminophenylphosphate* in 1 studies

Other Studies

1 other study(ies) available for pulmicort and 4-aminophenylphosphate

Article	Year
Determination of drugs in biosamples at picomolar concentrations using competitive ELISA with electrochemical detection: application to steroids. Journal of pharmaceutical and biomedical analysis, 1993, Volume: 11, Issue:6 A competitive ELISA with electrochemical detection in a flow injection system (FIA) has been developed for determinations of the steroid drug budesonide in biological samples. Plasma samples were cleaned from interfering and cross-reacting compounds by two pretreatment steps consisting of a solid-phase extraction and a liquid chromatography fractionation. The enzyme label was alkaline phosphatase, which was used with p-aminophenyl phosphate (PAPP) as a substrate. The product, p-aminophenol, was detected electrochemically at a glassy carbon electrode at 250 mV (vs Ag/AgCl). The limited stability of both the substrate and the product influenced the performance of the method and had to be taken into account in the procedure by a normalization with time. Budesonide could be quantified in plasma samples down to 10 pM. The major sensitivity-limiting factor was the amperometric background response, probably due to spontaneous hydrolysis of PAPP to p-aminophenol. Topics: Alkaline Phosphatase; Aminophenols; Aniline Compounds; Budesonide; Chromatography, Liquid; Cross Reactions; Electrochemistry; Electrodes; Enzyme-Linked Immunosorbent Assay; Flow Injection Analysis; Glucocorticoids; Humans; Organophosphorus Compounds; Pregnenediones; Sensitivity and Specificity	1993

Article

Year

Determination of drugs in biosamples at picomolar concentrations using competitive ELISA with electrochemical detection: application to steroids.

Journal of pharmaceutical and biomedical analysis, 1993, Volume: 11, Issue:6

A competitive ELISA with electrochemical detection in a flow injection system (FIA) has been developed for determinations of the steroid drug budesonide in biological samples. Plasma samples were cleaned from interfering and cross-reacting compounds by two pretreatment steps consisting of a solid-phase extraction and a liquid chromatography fractionation. The enzyme label was alkaline phosphatase, which was used with p-aminophenyl phosphate (PAPP) as a substrate. The product, p-aminophenol, was detected electrochemically at a glassy carbon electrode at 250 mV (vs Ag/AgCl). The limited stability of both the substrate and the product influenced the performance of the method and had to be taken into account in the procedure by a normalization with time. Budesonide could be quantified in plasma samples down to 10 pM. The major sensitivity-limiting factor was the amperometric background response, probably due to spontaneous hydrolysis of PAPP to p-aminophenol.

Topics: Alkaline Phosphatase; Aminophenols; Aniline Compounds; Budesonide; Chromatography, Liquid; Cross Reactions; Electrochemistry; Electrodes; Enzyme-Linked Immunosorbent Assay; Flow Injection Analysis; Glucocorticoids; Humans; Organophosphorus Compounds; Pregnenediones; Sensitivity and Specificity

1993