pulegone and carvone

The study compares the effect of two concentrations (0.1% and 1%) of plant material on repelling two dangerous stored pests -. Laboratory tests were carried out at 29±1 °C with 60±5% relative humidity (RH). Repellence of insects were noted after 1, 2, 3, 4, 5, 24 and 48 h.. A lower dose of essential oils and some of the tested compounds caused a stronger repellence in the specified species of beetles. In the case of R.. The most interesting results were obtained concerning the relationship: the lower tested substance concentration caused a much stronger repellent effect of R.

Topics: Animals; Coleoptera; Insecticides; Oils, Volatile; Weevils

A novel automated method was developed for the quantitative determination of nine terpenoids that could contribute to the minty notes of red wine bouquet. The method couples headspace SPME-Arrow extraction with GC-MS/MS analysis. PDMS/DVB fiber was chosen for the extraction and an ionization energy of 30 eV permitted to optimize the analyte detection. The optimal sample preparation consists of a two-fold dilution of the wine sample with addition of 4 g of sodium chloride while the most suitable extraction conditions take place at 50 °C for 1 h. The method shows good linearity, intraday variations between 2 and 25%, interday variations between 7 and 23% and recoveries between 80 and 119%. The method exhibits the required low detection (between 3 and 60 ng/L) and quantification (between 6 ng/L and 200 ng/L) limits. These limits have permitted the quantification of the pool of minty terpenoids in fourteen red Bordeaux wines.

Topics: Cyclohexane Monoterpenes; Dimethylpolysiloxanes; Eucalyptol; Gas Chromatography-Mass Spectrometry; Lactones; Limonene; Mentha; Menthol; Odorants; Polyvinyls; Solid Phase Microextraction; Tandem Mass Spectrometry; Terpenes; Wine

The study was aimed to investigate essential oil chemical composition (gas chromatography/flame ionization detection [GC-FID] and gas chromatography/mass spectrometry [GC-MS]) and antioxidant (1,1-diphenyl-2-picrylhydrazyl free radical (DPPH) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate [ABTS] assays) and antimicrobial (Gram-positive and Gram-negative bacteria, fungi, and yeast) activities of essential oils extracted from leaves of Mentha longifolia L. and Mentha viridis. GC-MS analysis revealed that M. longifolia was constituted by pulegone (54.41%) as a major component followed by isomenthone (12.02%), 1,8-cineole (7.41%), borneol (6.85%), and piperitenone oxide (3.19%). M. viridis was rich in carvone (50.47%), 1,8-cineole (9.14%), and limonene (4.87%). The antioxidant activity by ABTS assay showed IC(50) values of 476.3 +/- 11.7 and 195.1 +/- 4.2 mg/L for M. longifolia and M. viridis, respectively, the DPPH assays have resulted in a moderate IC(50) (>8000 mg/L and 3476.3 +/- 133 mg/L for M. longifolia and M. viridis, respectively). Antimicrobial activity showed that Listeria monocytogenes and Klebsiella pneumoniae bacteria were more inhibited by the 2 essential oils tested. Escherichia coli was least susceptible. A strong activity was also observed on fungi and yeasts. Carvone, thymol, and piperitone oxide have not been detected in Tunisian M. longifolia. Camphor is reported for the 1st time for M. viridis. Antioxidant and antibacterial activities were correlated to chemical composition.

Topics: Anti-Infective Agents; Antioxidants; Cyclohexane Monoterpenes; Distillation; Free Radical Scavengers; Fungi; Gas Chromatography-Mass Spectrometry; Gram-Negative Bacteria; Gram-Positive Bacteria; Mentha; Microbial Sensitivity Tests; Monoterpenes; Oils, Volatile; Plant Leaves; Species Specificity; Yeasts