prostaglandin-d2 and methyl-gallate

Methyl gallate (MG) is a medicinal herbal product that is isolated from Paeonia lactiflora that inhibits cyclooxygenase-2 (COX-2) dependent phases of prostaglandin D2 (PGD2) generation in bone marrow-derived mast cells (BMMC) in a concentration-dependent manner with an IC50 values of 17.0 microM. This compound also found inhibited the COX-2-dependent conversion of the exogenous arachidonic acid to PGD2 in a dose-dependent manner with an IC50 values of 19.0 microM, using a COX enzyme assay kit. However, at concentrations up to 80 microM, MG did not inhibit COX-2 protein expression in BMMC, indicating that MG inhibits COX-2 activity directly. Furthermore, MG consistently inhibited the production of leukotriene C4 (LTC4) in a dose dependent manner, with an IC50 value of 5.3 microM. These results demonstrate that MG has a dual cyclooxygenase-2/5-lipoxygenase inhibitory activity, which might provide the basis for novel anti-inflammatory drugs.

Topics: Animals; Arachidonate 5-Lipoxygenase; Arachidonic Acid; Bone Marrow Cells; Cell Line, Tumor; Cyclooxygenase 2; Cyclooxygenase Inhibitors; Dose-Response Relationship, Drug; Enzyme Inhibitors; Gallic Acid; Interleukin-10; Leukotriene C4; Lipopolysaccharides; Male; Mast Cells; Mice; Mice, Inbred BALB C; Molecular Structure; Paeonia; Plant Extracts; Plant Leaves; Plant Stems; Prostaglandin D2; Stem Cell Factor