prolylglycine and prolylleucine

To study the chemical constituents of n-butanol extract in the broth of Myrothecium verrucaria.. The chemical constituents were isolated,purified and identified by various chromatographic and spectral techniques.. 14 compounds were isolated and identified from n-butanol extracts of Myrothecium verrucaria broth. They were identified as cyclo-( Pro-Phe)( 1),cyclo-( 4-OH-Pro-Phe)( 2),cyclo-( 4-OH-Pro-Leu)( 3),cyclo-( Ala-Pro)( 4),cyclo-( 4-methyl-Pro-9-propyl-Gly)( 5),cyclo-( Pro-Gly)( 6),cyclo-( Phe-Gly)( 7),cyclo-( Leu-Leu)( 8),N-acetyl tryptamine( 9),N-( 2-phenylethyl) acetamide( 10),N-( 2-hydroxyphenethyl) acetamide( 11),N-( 4-hydroxyphenethyl) acetamide( 12),uracil( 13) and thymine( 14).. Compounds 1 ~ 14 are obtained from the strain Myrothecium verrucaria for the first time.

Topics: 1-Butanol; Dipeptides

Dipeptide (Pro-Gly and Pro-Leu) Zinc(II) complexes 1 and 2 were designed and synthesized for potential use as cancer chemotherapeutic agents. In order to augment the DNA recognition of metallonuclease activity, zinc metal ion was tethered to peptide motif to carry out DNA site specific hydrolytic cleavage. The structural formulation of the complexes 1 and 2 was done by elemental analysis, spectroscopic methods (IR, NMR, electronic) and molar conductance measurements. Their in vitro DNA binding profile was investigated by UV-vis titrations, fluorescence titrations and circular dichroism which revealed that these complexes bind to CT DNA by electrostatic interactions via groove binding mode. Zn(II) Pro-Gly complex 1 showed greater binding affinity to CT DNA as compared to the Zn(II) Pro-Leu complex 2 due to steric constraints in the latter. The supercoiled pBR322 DNA cleavage activity of complex 1, ascertained by gel electrophoresis demonstrated efficient DNA cleaving ability via hydrolytic mechanistic pathway. Further, the molecular docking studies confirmed that complex 1 bind to the minor groove of DNA having AT-rich sequences with relative binding energy of -196.72kJmol(-1).

Topics: Animals; Cattle; Chemistry Techniques, Synthetic; Dipeptides; DNA; DNA Cleavage; Ethidium; Hydrolysis; Models, Molecular; Nucleic Acid Conformation; Organometallic Compounds; Zinc

A rapid and accurate method is described for the determination of prolyl peptides in urine, with specific reference to the dipeptide prolylhydroxyproline, and free hydroxyproline and proline. Free amino acids and peptides were isolated from urine on cation-exchange minicolumns, and free imino acids and prolyl-N-terminal peptides were selectively derivatized with 4-chloro-7-nitrobenzofurazan, after reaction of amino acids and N-terminal aminoacyl peptides with o-phthalaldehyde. The highly fluorescent adducts of imino acids and prolyl peptides were separated on a Spherisorb ODS 2 column by isocratic elution for 12 min using as mobile phase 17.5 mM aqueous trifluoracetic acid solution containing 12.5% acetonitrile (eluent A), followed by gradient elution from eluent A to 40% of 17.5 mM aqueous trifluoroacetic acid solution containing 80% acetonitrile in 20 min. Analytes of interest, in particular the dipeptide prolylhydroxyproline, can be easily quantified by fluorimetric detection (epsilon ex = 470 nm, epsilon em = 530 nm) without interference from primary amino-containing compounds.

Topics: Chromatography, High Pressure Liquid; Dipeptides; Humans; Hydroxyproline; Peptides; Reference Values; Spectrophotometry, Ultraviolet