procyanidin-b1 and proanthocyanidin

To confirm the absorption of proanthocyanidin (PA) into the human body, four healthy adults were administered 2.0 g of PA-rich grape seed extract (GSE). Blood were drawn before intake and 2 h after intake. Through the enzymatic treatment of sulfatase and beta-glucuronidase, blood samples were analyzed by HPLC coupled with mass-spectrometry (LC/MS). Procyanidin B1 [epicatechin-(4beta-->8)-catechin] was detected in human serum 2 h after intake. Its concentration was 10.6 +/- 2.5 nmol/l.

Topics: Antioxidants; Biflavonoids; Catechin; Chromatography, High Pressure Liquid; Electrochemistry; Humans; Intestinal Absorption; Mass Spectrometry; Plant Extracts; Proanthocyanidins; Seeds; Vitis

Aimed at exploring the dentin biomodification potential of proanthocyanidins (PACs) for the development of dental biomaterials, this study reports the phytochemical and dental evaluation of nine B-type PACs from grape seed extract (GSE). Out of seven isolated dimers (

Topics: Biopolymers; Biotin; Proanthocyanidins

Shelf life of commercial cranberry syrup irradiated with gamma radiation at a rate of 5 kGy and stored for 6 months at 25 °C and 60% relative humidity (RH) and under accelerated stability conditions was investigated. High-performance liquid chromatography coupled to electrospray ionisation quadrupole-time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS) was used to characterise cranberry syrup. Afterwards, these compounds were quantified by HPLC-ESI-QTOF-MS and 4-dimethylaminocinnamaldehyde (DMAC) assay. A significant increase in the content of procyanidin B isomer 1 (from 4.4 to 7.0 μg/ml) and procyanidin A2 (from 83 to 93 μg/ml) was observed after irradiation and compared with the non-irradiated syrup. Procyanidin B isomers and prodelphinidin were stable at 25 °C during the first month of storage, whereas quercetin and some derivatives remained constant for 3 months of storage at this temperature. In short, after gamma-irradiation in dose of 5 kGy, most compounds were highly stable for a month at 25 °C.

Topics: Biflavonoids; Catechin; Chromatography, High Pressure Liquid; Cinnamates; Dose-Response Relationship, Radiation; Food Irradiation; Food Storage; Gamma Rays; Limit of Detection; Phenols; Proanthocyanidins; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry; Vaccinium macrocarpon

Several procyanidins with different degrees of polymerization (dimers, a trimer and a polymer) and extracted from different natural sources were found to be non-mutagenic in the Salmonella mutagenesis assay system. A mutagenic impurity in procyanidin B-4 was isolated by means of reversed-phase high-performance liquid chromatography (HPLC) and identified as rutin with UV spectrometry, co-chromatography on reversed-phase HPLC and gas chromatography-mass spectrometry.

Topics: Anthocyanins; Biflavonoids; Catechin; Chromatography, High Pressure Liquid; Gas Chromatography-Mass Spectrometry; Mutagens; Proanthocyanidins; Spectrophotometry, Ultraviolet; Structure-Activity Relationship