povidone-iodine and iodoform

Topics: Anti-Infective Agents, Local; Bandages; Electroencephalography; Humans; Hydrocarbons, Iodinated; Hyperthyroidism; Magnetic Resonance Imaging; Male; Middle Aged; Nervous System Diseases; Povidone-Iodine; Shock, Septic; Thyrotropin; Thyroxine

The antibacterial and antifungal effects of medicated gutta-percha (MGP) over different time periods have not been evaluated. The aim of this study was to evaluate and compare the antimicrobial and antifungal effectiveness of MGP and regular gutta-percha cones over different time periods using the disk diffusion method.. Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli were spread onto Mueller-Hinton agar, and Candida albicans was spread onto Sabouraud agar supplemented with glucose. Same-size MGP cones, conventional gutta-percha cones and disks impregnated with povidone-iodine were placed on the inoculated plates. The dishes were incubated at 37 degrees C aerobically. Growth inhibition zones were inspected and measured at 24, 48 and 72 hours. For each strain, experiments were performed in triplicate. The Kruskal-Wallis test was used to compare k independent samples.. The disks impregnated with povidone-iodine inhibited all bacterial strains for up to 72 hours. No inhibition zones were seen around regular gutta-percha cones. MGP inhibited the growth of all bacteria over 24 hours, but in some cases these effects did not continue over longer periods. Specifically, the antimicrobial properties of MGP cones against E. coli and P. aeruginosa disappeared by 48 and 72 hours. Regardless of time and bacterial strain, MGP was statistically more effective than regular gutta-percha (p < 0.05). Povidone-iodine and MGP were effective against C. albicans for up to 72 hours, but regular gutta-percha exhibited no antifungal activity.. The antimicrobial and antifungal characteristics of MGP may offer additional advantages over conventional gutta-percha.

Topics: Anti-Infective Agents, Local; Bacteria; Candida albicans; Drug Combinations; Enterococcus faecalis; Escherichia coli; Gutta-Percha; Hydrocarbons, Iodinated; Povidone-Iodine; Pseudomonas aeruginosa; Root Canal Filling Materials; Staphylococcus aureus; Statistics, Nonparametric; Time Factors

To determine whether 'MGP' gutta-percha (Westport, CT, USA), a commercially available gutta-percha containing iodoform, inhibits the growth of potential endodontic pathogens.. Inocula of Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus sanguis, Fusobacterium nucleatum and Actinomyces odontolyticus were spread onto the surface of agar plates. 'MGP' gutta-percha cones presoaked in sterile water were transferred to the inoculated agar and incubated at 37 degrees C aerobically or anaerobically as required for optimal growth. Identical studies were performed using iodoform-free gutta-percha and sterile paper disks saturated with 10% povidone-iodine. Following incubation, zones of inhibition around the 'MGP' gutta-percha, iodoform-free gutta-percha and disks were evaluated.. Povidone-iodine inhibited all the strains. Iodoform-free gutta-percha inhibited S. sanguis and A. odontolyticus. 'MGP' gutta-percha inhibited S. aureus, S. sanguis, A. odontolyticus and F. nucleatum. Neither iodoform-free gutta-percha nor 'MGP' gutta-percha inhibited growth of E. faecalis, E. coli or P. aeruginosa.. Compared to iodoform-free gutta-percha, iodoform-containing 'MGP' gutta-percha had an inhibitory effect in vitro on S. aureus and F. nucleatum, but not on E. faecalis, E. coli or P. aeruginosa.

Topics: Actinomyces; Anti-Infective Agents; Anti-Infective Agents, Local; Bacteria; Enterococcus faecalis; Escherichia coli; Fusobacterium nucleatum; Gutta-Percha; Humans; Hydrocarbons, Iodinated; Povidone-Iodine; Pseudomonas aeruginosa; Root Canal Filling Materials; Staphylococcus aureus; Streptococcus sanguis; Temperature