potassium-thiocyanate and acetyl-chloride

potassium-thiocyanate has been researched along with acetyl-chloride* in 1 studies

Other Studies

1 other study(ies) available for potassium-thiocyanate and acetyl-chloride

Article	Year
A method for preparation of amino acid thiohydantoins from free amino acids activated by acetyl chloride for development of protein C-terminal sequencing. Analytical biochemistry, 1997, Jul-01, Volume: 249, Issue:2 A novel and efficient method to prepare amino acid thiohydantoins, which are required as reference standards for development of C-terminal protein sequencing, is reported. Amino acid thiohydantoins were prepared using a straightforward method involving reaction of 20 free amino acids with acetyl chloride as activating reagent and trimethylsilyl isothiocyanate (TMS-ITC) as derivatizing reagent. The products were characterized by HPLC, uv spectra, amino acid analysis, MS, and NMR. Different reaction conditions were investigated and the chemical mechanism of the formation of amino acid thiohydantoins was illustrated. Topics: Acetates; Amino Acids; Aminoacylation; Chlorides; Chromatography, High Pressure Liquid; Isothiocyanates; Magnetic Resonance Spectroscopy; Mass Spectrometry; Proteins; Sequence Analysis; Spectrophotometry, Ultraviolet; Thiocyanates; Thiohydantoins; Trimethylsilyl Compounds	1997

Article

Year

A method for preparation of amino acid thiohydantoins from free amino acids activated by acetyl chloride for development of protein C-terminal sequencing.

Analytical biochemistry, 1997, Jul-01, Volume: 249, Issue:2

A novel and efficient method to prepare amino acid thiohydantoins, which are required as reference standards for development of C-terminal protein sequencing, is reported. Amino acid thiohydantoins were prepared using a straightforward method involving reaction of 20 free amino acids with acetyl chloride as activating reagent and trimethylsilyl isothiocyanate (TMS-ITC) as derivatizing reagent. The products were characterized by HPLC, uv spectra, amino acid analysis, MS, and NMR. Different reaction conditions were investigated and the chemical mechanism of the formation of amino acid thiohydantoins was illustrated.

Topics: Acetates; Amino Acids; Aminoacylation; Chlorides; Chromatography, High Pressure Liquid; Isothiocyanates; Magnetic Resonance Spectroscopy; Mass Spectrometry; Proteins; Sequence Analysis; Spectrophotometry, Ultraviolet; Thiocyanates; Thiohydantoins; Trimethylsilyl Compounds

1997