potassium-permanganate and sodium-thiosulfate

The purpose of this study was to find a suitable method of labelling cartilage samples for the measurement of distraction distances in biomechanical testing.. Samples of bovine cartilage were labelled using five different methods: hydroquinone and silver nitrate (AgNO3), potassium permanganate (KMnO4) with sodium thiosulphate (Na2S2O3), India ink, heat, and laser energy. After the labelling, we analysed the cartilage samples with regard to cytotoxity by histochemical staining with ethidiumbromide homodimer (EthD-1) and calcein AM. Furthermore, we tested cartilages labelled with India ink and heat in a T-peel test configuration to analyse possible changes in the mechanical behaviour between marked and unlabelled samples.. Only the labelling methods with Indian ink or a heated needle showed acceptable results in the cytotoxity test with regard to labelling persistence, accuracy, and the influence on consistency and viability of the chondrocytes. In the biomechanical T-peel configuration, heat-labelled samples collapsed significantly earlier than unlabelled samples.. Labelling bovine cartilage samples with Indian ink in biomechanical testing is a reliable, accurate, inexpensive, and easy-to-perform method. This labelling method influenced neither the biomechanical behaviour nor the viability of the tissue compared to untreated bovine cartilage.

Topics: Animals; Biomechanical Phenomena; Carbon; Cartilage, Articular; Cattle; Cell Survival; Chondrocytes; Coloring Agents; Hot Temperature; Hydroquinones; Lasers; Materials Testing; Potassium Permanganate; Reproducibility of Results; Silver Nitrate; Staining and Labeling; Thiosulfates

A novel rapid and sensitive analytical method, enhanced chemiluminescence with flow-injection sampling, is described for determination of ciprofloxacin. The method is based on the chemiluminescence reaction of the potassium permanganate-sodium thiosulfate-ciprofloxacin system. An enhanced chemiluminescence reaction was developed, and optimum conditions for CL emission were investigated. The chemiluminescence intensity was linearly dependent on ciprofloxacin concentration in the range 1.0 x 10(-8)-1.0 x 10(-5) g mL(-1). The detection limit was 4 x 10(-9) g mL(-1). The relative standard deviation was 1.8% for eleven measurements of 2.0 x 10(-7) g mL(-1) ciprofloxacin standard solution. The new method enables simple, sensitive, and rapid determination of ciprofloxacin and has been successfully used for determination of ciprofloxacin in biological fluids and in ciprofloxacin hydrochloride tablet and injection.

Topics: Antioxidants; Body Fluids; Ciprofloxacin; Flow Injection Analysis; Humans; Luminescent Measurements; Pharmaceutical Preparations; Potassium Permanganate; Sensitivity and Specificity; Thiosulfates

Treatment of pyo-inflammatory diseases in patients with diabetes mellitus should be complex, individual and controlled. Obligatory total and local examinations of the organism should be performed with the involvement of cytological, histological bacteriological methods and with determination of alterations of the carbohydrate, protein, water-salt, electrolytic metabolism, acid-base state and non-specific resistance of the organism. It was shown that diadynamophoresis of proteolytic enzymes, sodium thiosulfate, potassium permanganate in the treatment of pyo-destructive diseases in 362 patients with diabetes mellitus accelerated necrolysis of tissues, shortened time of cleaning the wound and thus made the period of treatment at the hospital shorter.

Topics: Animals; Diabetes Complications; Diabetes Mellitus, Experimental; Drug Therapy, Combination; Humans; Iontophoresis; Male; Peptide Hydrolases; Potassium Permanganate; Rats; Skin Diseases, Infectious; Thiosulfates

Topics: Dermabrasion; Humans; Potassium Permanganate; Tattooing; Thiosulfates