potassium-permanganate and sodium-sulfite

A simple and selective method to determine norfloxacin using an optical flow-through sensor has been developed. The present sensor was prepared by packing anionic ion exchange resin in a glass tube, followed by introducing KMnO(4) solution to the glass tube for immobilization on resin. The optical sensor is based on the emission intensity from the Tb(III) solution sensitized by norfloxacin. The excitation of norfloxacin occurred by the chemiluminescence from the reaction of KMnO(4) and Na(2)SO(4) solutions. The effects of pH, concentration of Tb(III) ion, KMnO(4) and Na(2)SO(4) solutions and flow rate of the norfloxacin solution on the chemiluminescence intensity were studied to find the optimum experimental conditions. The emission intensity increased linearly with increasing norfloxacin concentration from 1.0 x 10(-3) to 1.0 x 10(-8) M and the detection limit (3sigma) was 8.7 x 10(-9). The applicability of the present method was demonstrated by determination of norfloxacin in various pharmaceutical preparations and serum sample.

Topics: Anti-Bacterial Agents; Biosensing Techniques; Hydrogen-Ion Concentration; Luminescence; Luminescent Measurements; Molecular Structure; Norfloxacin; Potassium Permanganate; Reproducibility of Results; Sensitivity and Specificity; Sulfites; Terbium

A novel method was developed for the simultaneous determination of tetracycline antibiotic (TCA) residues such as oxytetracycline (OTC), tetracycline (TC), and metacycline (MTC) by high-performance liquid chromatography (HPLC) coupled with chemiluminescence (CL) detection. The procedure was based on the chemiluminescent enhancement by TCAs of the potassium permanganate-sodium sulfite-beta-cyclodextrin system in a phosphoric acid medium. The separation was carried out with an isocratic elution using a mixture of acetonitrile and 0.001 M phosphoric acid. For the three TCAs, the detection limits at a signal-to-noise of 3 ranged from 0.9 to 5.0 ng/ml. The relative standard deviations for the determination of TCAs ranged from 3.1 to 7.4% within a day (n=11) and ranged from 2.2 to 8.6% in 3 days (n=9), respectively. The method was successfully applied to the determination of TCA residues in honey samples. The possible mechanism of the CL reaction was also discussed.

Topics: beta-Cyclodextrins; Chromatography, High Pressure Liquid; Honey; Luminescent Measurements; Methacycline; Oxytetracycline; Potassium Permanganate; Reproducibility of Results; Sulfites; Tetracyclines

A molecular imprinted polymer of morphine was synthesized. Using the imprinted polymer as the recognition material, sodium sulfite as the protective agent, and a potassium permanganate-morphine chemiluminescence system as the detection system, a molecular imprinting-chemiluminescence method for the determination of morphine was established. The linear response range of this method was 5.0 x 10-9-1.0 x 10-6 g/mL (r = 0.9981) and the detection limit was 2 x 10-9 g/mL. The coefficient of variation for 1.0 x 10-7 g/mL morphine solution was 2.8% (n = 9). This method was applied to the determination of morphine in the urine of the heroin abusers with satisfactory results.

Topics: Calibration; Humans; Luminescent Measurements; Morphine; Polymers; Potassium Permanganate; Sensitivity and Specificity; Substance Abuse Detection; Sulfites

To study the sensitizing effect of acemetacin (ACE) on the weak chemiluminescent (CL) reaction of KMnO4 with sulfite and establish a fast and convenient method for CL detection of ACE.. Using the sensitizing effect of ACE on KMnO4-Na2SO3 system and flow injection technique to determine the concentration of ACE.. Under optimal conditions, the CL intensity of 1.0 x 10(-2) mol x L(-1) H3PO4 - 5.0 x 10(-5) mol x L(-1) KMnO4 - 4.0 x 10(-4) mol x L(-1) Na2SO3 was proportional to the concentration of ACE ranging from 1.0 x 10(-7) to 1.0 x 10(-5) mol x L(-1). The detection limit of ACE was 6.9 x 10(-8) mol x L(-1) at 3sigma. Satisfactory results were obtained for determination of ACE at 2.5 x 10(-6) mol x L(-1).. The present method showed good precision, high sensitivity and selectivity and could be used for fast and convenient detection of ACE. It would be of significance to the clinical and pharmacological study of acemetacin.

Topics: Flow Injection Analysis; Indomethacin; Luminescent Measurements; Potassium Permanganate; Sulfites