plasminogen-activator-inhibitor-2 and bis(3--5-)-cyclic-diguanylic-acid

Aeromonas is a ubiquitous gram-negative bacterium that persists in the environment. It is shown that all isolates of persistent Aeromonas clones show strong biofilm formation ability. C-di-GMP regulates biofilm formation in many bacteria. To investigate the impact of c-di-GMP signaling, we introduced heterologous GGDEF and EAL domain proteins from Salmonella Typhimurium to an Aeromonas veronii biovar sobria strain. Overexpression of the GGDEF domain protein AdrA increased c-di-GMP concentration and biofilm formation and reduced motility. Production of the quorum-sensing signaling molecule C4-homoserine lactone and adhesion to aquatic plant duckweed and amoeba surfaces were enhanced. On the other hand, overexpression of the EAL domain protein YhjH decreased biofilm formation and increased motility.

Topics: 4-Butyrolactone; Aeromonas; Amoeba; Animals; Araceae; Bacterial Adhesion; Bacterial Proteins; Biofilms; Cyclic GMP; Protein Structure, Tertiary; Quorum Sensing; Salmonella typhimurium; Signal Transduction