plantamajoside and acteoside

In the present study, ternary deep eutectic solvent-based ultrasound-assisted extraction was developed for the efficient extraction of plantamajoside, acteoside, quercetin and kaempferol from Plantago asiatica L.. Six kinds of choline chloride-based ternary deep eutectic solvents (TDESs) were prepared as potential extraction solutions. In order to obtain optimal extraction efficiency, a series of extraction conditions were investigated by single-factor test and orthogonal test.. The extraction efficiency of choline chloride/lactic acid/ethylene glycol (ChCl-LA-EG) was much higher than that of other TDESs. ChCl-LA-EG-11 synthesised with choline chloride, lactic acid and ethylene glycol (1:4:2) was considered to have a higher extraction efficiency. The optimal ultrasound-assisted extraction conditions were as follows: water content in ChCl-LA-EG-11, 50%; extraction temperature, 70°C; ratio of solid/liquid, 20 mg/mL; ultrasonic power, 60 W; extraction time, 35 min; pH of the solution, 8. Under the optimal extraction conditions, the extraction efficiencies of plantamajoside, acteoside, quercetin and kaempferol were 3.83 ± 0.41, 4.23 ± 0.45, 0.56 ± 0.15 and 0.19 ± 0.08 mg/g, respectively. The extraction efficiency of the total target components was 9.21 ± 0.63 mg/g, which was much higher than that of conventional solvents (water, methanol, ethanol, 50% methanol, 50% ethanol). The target components were isolated efficiently from the TDES solution by an AB-8 macroporous resin column with a recovery rate of 95.6%.. This study demonstrated that TDESs possessed excellent physical and chemical properties and had enormous potential for active component extraction of traditional Chinese medicinal materials.

Topics: Catechols; Deep Eutectic Solvents; Glucosides; Kaempferols; Phenols; Plantago; Quercetin; Solvents

Plantago asiatica is a medicinal and dietary plant rich in polyphenolic compounds such as phenylpropanoid glycosides plantamajoside and acteoside. The aims of the present study were to develop a new and sensitive method for simultaneous determination of plantamajoside and acteoside and investigate their pharmacokinetic properties in rats. A sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method with multiple-reaction monitoring (MRM) mode was employed for quantification of two analytes in rat plasma. The calibration curve was linear over the range of 0.2-200ng/ml with correlation coefficient greater than 0.9983 for both analytes. The accuracy of plantamajoside and acteoside were between -4.2% and 8.1%, -3.8% and 8.9% relative error, respectively. Precision for the two analytes ranged from 2.7 to 10.2% relative standard deviation. The pharmacokinetic results showed plantamajoside and acteoside were quickly absorbed in rat with the time to maximum plasma concentration 16.7±2.8 and 13.3±2.8min, respectively. The elimination constants were 0.28±0.01 1h(-1) for plantamajoside and 0.47±0.03 1h(-1) for acteoside. The developed method and the pharmacokinetic data provide a basis for further studies on bioactivity of P. asiatica.

Topics: Animals; Catechols; Chromatography, Liquid; Glucosides; Male; Phenols; Plant Extracts; Plantago; Rats; Rats, Sprague-Dawley; Tandem Mass Spectrometry