pituitrin and phorbol-12-13-didecanoate

Intracerebroventricular (icv) injection of transient receptor potential vanilloid 4 (TRPV4) agonists 4α-phorbol-12, 13-didecanoate (4α-PDD) and GSK101690A increased urinary excretion under the physiological condition. TRPV4 antagonists ruthenium red and HC-067047 significantly blocked increased urinary volume after intragastric administration of water and 4α-PDD-induced diuresis. Administration of the TRPV4 agonists did not significantly change the plasma concentration of vasopressin or atrial natriuretic factor. Pretreatment with indomethacin inhibited the diuresis induced by 4α-PDD. Moreover, icv injection of prostaglandin (PG) F

Topics: Animals; Atrial Natriuretic Factor; Dinoprost; Diuresis; Drinking; Homeostasis; Indomethacin; Male; Morpholines; Phorbol Esters; Pyrroles; Rats; Rats, Wistar; Ruthenium Red; TRPV Cation Channels; Urination; Vasopressins