pituitrin and phenylalanyl-leucyl-arginyl-phenylalanine

The two vasopressin-like immunoreactive (VPLI) neurons of the locust, Locusta migratoria, have cell bodies in the suboesophageal ganglion and extensive arborizations throughout the CNS. One of the two peptides responsible for AVP-like immunoreactivity is a vasopressin-related peptide with putative 'diuretic hormone' properties. These neurons also have FLRF-like immunoreactivity, probably due to the FMRF-amide-related peptide. SchistoFLRF-amide, isolated from Schistocerca gregaria. This peptide has cardioinhibitory activity and a dual potentiation/inhibition of slow motoneuron induced muscle-twitch tension. Although haemolymph AVP-like peptide titre fluctuates under various conditions, the mechanism that regulates neurohaemal release of this peptide is not understood. Very little is known of the release of SchistoFLRF-amide. We have used intracellular recording from VPLI neurons in vivo to reveal synaptic inputs that lead to changes in their level of spiking activity, and probably, release of both the AVP-like peptides and SchistoFLRF-amide. This pair of neurosecretory cells has a major, common excitatory input whose sustained rate of activity is inversely related to light intensity; VPLI spiking activity, driven by this input, is greater in the dark than in light. This input is from a pair of descending brain interneurons. Their light-sensitivity persists after ablation of compound eyes, optic lobes and ocelli, showing them to be part of an extra-ocular photoreceptor system. Attempts to record from, and individually stain, the descending neuron have been unsuccessful, although its axon location and diameter in the circumoesophageal connective have been determined. Possible locations for its cell body have been identified; one region, close to the pars intercerebralis, is known to be photosensitive in some insects. Mechanosensory stimuli also lead to brief increases in VPLI spiking activity via the descending interneuron, though this modality rapidly habituates. We detect no changes in VPLI spiking activity that consistently correlate with the osmolality of perfusion salines; such changes might have been expected from their previously proposed role in water homeostasis. Alternative roles for VPLI cells are discussed.

Topics: Animals; Antibodies; Axons; Brain; Chemoreceptor Cells; Grasshoppers; Homeostasis; Immunohistochemistry; Light; Mechanoreceptors; Neurons; Oligopeptides; Osmolar Concentration; Vasopressins

The distribution of Phe-Leu-Arg-Phe (FLRF)-like immunoreactivity is described in the brain and in the ganglia of the ventral nerve cord of the locust Schistocerca gregaria. A single homologous pair of immunoreactive cell bodies occurs ventrally and medially in the suboesophageal ganglion. Each cell sends a process dorsally which bifurcates into anteriorly and posteriorly running neurites. The single anterior neurite passes along the circumoesophageal connectives to the brain where it ascends in a posterior running tract, giving off branches to innervate the tritocerebral neuropile and ending in an extensive network of highly varicose immunoreactive processes in the protocerebral neuropile. No processes are seen in the optic lobes or associated with the structured neuropiles of the muschroom bodies. The single posterior neurite from each cell passes into the suboesophageal-prothoracic connectives. It runs in the lateral dorsal tract of each ganglion in the ventral nerve cord as a highly varicose process and in each ganglion gives rise to an ipsilateral network of varicose processes in the dorsal and lateral neuropiles. In the seventh and terminal abdominal ganglia the innervation pattern exhibits sexual dimorphism. Vasopressin-like immunoreactivity is co-localized in the same pair of suboesophageal neurones and their processes. A similar pair of ventral median neurones stains with both antibodies in the suboesophageal ganglion of another species of locust, Locusta migratoria. Although the basic distribution pattern of immunoreactive processes is similar in both species there are also marked species differences in the pattern.

Topics: Amino Acid Sequence; Animals; Female; FMRFamide; Grasshoppers; Immunohistochemistry; Invertebrate Hormones; Male; Molecular Sequence Data; Nervous System; Nervous System Physiological Phenomena; Neuropeptides; Oligopeptides; Sex Characteristics; Vasopressins