pituitrin and hydroxysuccinimidyl-4-azidobenzoate

pituitrin has been researched along with hydroxysuccinimidyl-4-azidobenzoate* in 1 studies

Other Studies

1 other study(ies) available for pituitrin and hydroxysuccinimidyl-4-azidobenzoate

Article	Year
A V1-vascular vasopressin antagonist suitable for radioiodination and photoaffinity labeling. American journal of hypertension, 1990, Volume: 3, Issue:6 Pt 1 We have previously characterized the V1-vascular arginine vasopressin (AVP) receptors of human platelets (Thibonnier M, J Biol Chem 1987;262: 10960-10964). We now report on a radiomonoiodinated and photoreactive V1-vascular AVP antagonist (V1-ag) to be used for the purification of human V1-vascular AVP receptors. The V1-ag, d(CH2)5Tyr(Me)Tyr(NH2)AVP was modified by radiomonoiodination of d(CH2)5-Tyr(Me)Tyr(NH2)AVP with the Iodogen technique, and derivatization of d(CH2)5Tyr(Me)Tyr[125I](NH2)-AVP with the photoreactive crosslinker, N-hydroxysuccinimidyl-4-azidobenzoate (HSAB) (each step included HPLC purification). In competition experiments, the affinity of these V1-ag for the human platelet AVP receptors remained excellent: [table: see text] Irreversible photoaffinity labeling of the platelet V1-vascular AVP receptor was successfully achieved by UV lamp exposure (365 nm, 20 min). Thus, AzBz-d(CH2)5Tyr(Me)Tyr[125I](NH2)AVP is a promising tool to use for the purification of human V1-vascular AVP receptors. Topics: Affinity Labels; Arginine Vasopressin; Azides; Blood Platelets; Cell Membrane; Humans; Iodine Radioisotopes; Vasopressins	1990

Article

Year

A V1-vascular vasopressin antagonist suitable for radioiodination and photoaffinity labeling.

American journal of hypertension, 1990, Volume: 3, Issue:6 Pt 1

We have previously characterized the V1-vascular arginine vasopressin (AVP) receptors of human platelets (Thibonnier M, J Biol Chem 1987;262: 10960-10964). We now report on a radiomonoiodinated and photoreactive V1-vascular AVP antagonist (V1-ag) to be used for the purification of human V1-vascular AVP receptors. The V1-ag, d(CH2)5Tyr(Me)Tyr(NH2)AVP was modified by radiomonoiodination of d(CH2)5-Tyr(Me)Tyr(NH2)AVP with the Iodogen technique, and derivatization of d(CH2)5Tyr(Me)Tyr[125I](NH2)-AVP with the photoreactive crosslinker, N-hydroxysuccinimidyl-4-azidobenzoate (HSAB) (each step included HPLC purification). In competition experiments, the affinity of these V1-ag for the human platelet AVP receptors remained excellent: [table: see text] Irreversible photoaffinity labeling of the platelet V1-vascular AVP receptor was successfully achieved by UV lamp exposure (365 nm, 20 min). Thus, AzBz-d(CH2)5Tyr(Me)Tyr[125I](NH2)AVP is a promising tool to use for the purification of human V1-vascular AVP receptors.

Topics: Affinity Labels; Arginine Vasopressin; Azides; Blood Platelets; Cell Membrane; Humans; Iodine Radioisotopes; Vasopressins

1990