piperidines and formamide

piperidines has been researched along with formamide* in 2 studies

Other Studies

2 other study(ies) available for piperidines and formamide

Article	Year
Chemical method for DNA sequence determination from the 5'-extremity on PCR amplified fragments. Nucleic acids research, 1994, Jan-11, Volume: 22, Issue:1 Topics: Base Sequence; Formamides; Molecular Sequence Data; Piperidines; Polymerase Chain Reaction; RNA, Ribosomal, 5S; Saccharomyces cerevisiae	1994
One-lane chemical sequencing of 3'-fluorescent-labeled DNA. Analytical biochemistry, 1993, Nov-01, Volume: 214, Issue:2 A method for DNA sequencing that combines limited chemical degradation of 3'-fluorescent-labeled DNA with densitometric analysis using an automatic sequencer is presented. The DNA sequence is determined in a single electrophoretic lane by monitoring the intensities of bands representing products of cleavage at the four bases obtained by solvolysis in hot aqueous piperidine (10%) followed by treatment with hot formamide. An application of the method for the detection of point mutations is reported. Topics: Autoanalysis; Base Sequence; Computer Systems; Densitometry; DNA; Fluorescence; Formamides; Lasers; Molecular Sequence Data; Piperidines; Point Mutation	1993

Article

Year

Chemical method for DNA sequence determination from the 5'-extremity on PCR amplified fragments.

Nucleic acids research, 1994, Jan-11, Volume: 22, Issue:1

Topics: Base Sequence; Formamides; Molecular Sequence Data; Piperidines; Polymerase Chain Reaction; RNA, Ribosomal, 5S; Saccharomyces cerevisiae

1994

One-lane chemical sequencing of 3'-fluorescent-labeled DNA.

Analytical biochemistry, 1993, Nov-01, Volume: 214, Issue:2

A method for DNA sequencing that combines limited chemical degradation of 3'-fluorescent-labeled DNA with densitometric analysis using an automatic sequencer is presented. The DNA sequence is determined in a single electrophoretic lane by monitoring the intensities of bands representing products of cleavage at the four bases obtained by solvolysis in hot aqueous piperidine (10%) followed by treatment with hot formamide. An application of the method for the detection of point mutations is reported.

Topics: Autoanalysis; Base Sequence; Computer Systems; Densitometry; DNA; Fluorescence; Formamides; Lasers; Molecular Sequence Data; Piperidines; Point Mutation

1993