piperidines and cycrimine

Due to the limitations of current antiviral therapies because of drug resistance and the emergence of new circulating viral strains, novel effective antivirals are urgently needed. Results of the previous drug repurposing by virtual screening of DrugBank revealed the anticholinergic drug cycrimine as a possible inhibitor of the influenza virus infection.. In this study we examined the potential antiviral activity of cycrimine in vitro.. The experimental results showed the anti-influenza activity of cycrimine against two different influenza A subtypes in cell culture.. The findings of this study suggest cycrimine as a potential therapeutic agent for influenza.

Topics: Animals; Cell Line; Cell Survival; Computer Simulation; Dogs; Drug Repositioning; Influenza A Virus, H1N1 Subtype; Influenza A Virus, H3N2 Subtype; Piperidines

A sensitive (50-100 pg/ml) method is described for the analysis of the anticholinergic drugs cycrimine, procyclidine and trihexyphenidyl by capillary gas chromatography with flame thermionic detection. Since these anticholinergic drugs are frequently administered in combination with antipsychotic medication for the treatment of mental illness, the potential interference by antipsychotic drugs in this assay was examined. No interference was observed from a series of antipsychotic drugs in the quantitation of cycrimine, procyclidine or trihexyphenidyl. The use of this technique to study trihexyphenidyl pharmacokinetics in man is described.

Topics: Antipsychotic Agents; Chromatography, Gas; False Positive Reactions; Flame Ionization; Humans; Parasympatholytics; Piperidines; Procyclidine; Pyrrolidines; Trihexyphenidyl

Cycrimine hydrochloride, an anti-Parkinson drug having central cholinolytic properties, was found to cause neuromuscular blockade in the isolated rat diaphragm preparation. The neuromuscular blockade by cycrimine remained unaffected in presence of neostigmine and tetraethylammonium. Lower concentrations of cycrimine potentiated the paralytic effect of d-tubocurarine. A similar paralytic effect of cycrimine was observed in the pharmacologically denervated rat diaphragm. Responses to acetylcholine administered by retrograde i.v. injection were found to be inhibited in presence of cycrimine. These results suggest that the neuromuscular blockade by cycrimine is produced by a postsynaptic direct effect on the muscle.

Topics: Acetylcholine; Animals; Antiparkinson Agents; Denervation; Diaphragm; Electric Stimulation; Female; Hemicholinium 3; Male; Muscle Contraction; Neostigmine; Phrenic Nerve; Piperidines; Rats; Tubocurarine

Topics: Biomedical Research; Humans; Motion Sickness; Muscarinic Antagonists; Piperidines; Procyclidine; Trihexyphenidyl

Topics: Humans; Nervous System Diseases; Parkinson Disease; Piperidines; Trihexyphenidyl

Topics: Cardiovascular Agents; Muscarinic Antagonists; Muscle Relaxants, Central; Parkinson Disease; Piperidines

Topics: Cardiovascular Agents; Muscle Relaxants, Central; Parkinson Disease; Parkinsonian Disorders; Piperidines