piperidines and 7-methylguanine

piperidines has been researched along with 7-methylguanine* in 2 studies

Other Studies

2 other study(ies) available for piperidines and 7-methylguanine

Article	Year
A new technique for determining the distribution of N7-methylguanine using an automated DNA sequencer. Carcinogenesis, 1991, Volume: 12, Issue:11 We have developed a method to determine rapidly the sequence specificity of DNA alkylation resulting from chemical treatment. The utility of this approach is demonstrated here in a study of the sequence specificity of alkylation by dimethylsulphate (DMS). The method is independent of the sequence chosen and makes use of the polymerase chain reaction (PCR) to generate a fluorescently labelled DNA target. In this study, a 302 bp segment of the Escherichia coli lacI gene was amplified and the product purified by liquid chromatography on a Mono Q column. This DNA was alkylated with DMS and treated with hot piperidine to produce single-strand breaks at sites of N7 alkylation. The distribution of the break points, and hence the position and extent of alkylation, were determined on an Applied Biosystems 370A automated DNA sequencer. Topics: Alkylation; DNA; DNA Damage; Escherichia coli; Gene Amplification; Guanine; Lac Operon; Molecular Sequence Data; Oligonucleotides; Piperidines; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Sulfuric Acid Esters	1991
Mechanism of DNA strand breakage by piperidine at sites of N7-alkylguanines. Biochimica et biophysica acta, 1986, Oct-16, Volume: 868, Issue:1 The volatile, secondary amine piperidine is used in the Maxam-Gilbert chemical method of DNA sequencing to create strand breaks in DNA at sites of damaged bases. As such it is often used in generalized studies of DNA damage to identify 'alkali-labile lesions'. We confirm the mechanism proposed by Maxam and Gilbert (Maxam, A. and Gilbert, W. (1980) Methods Enzymol. 65, 499-560) by which aqueous piperidine creates strand breaks at sites of N7-guanine alkylations: alkaline conditions catalyze rupture of the C8-N9 bond, forming a formamido-pyrimidine structure which is displaced from the ribose moiety by piperidine. In keeping with this mechanism, the tertiary amine, N-methylpiperidine, does not catalyze the formation of strand breaks in alkylated DNA. Our data confirm the prediction that high pH in and of itself will not create strand breaks at sites of N7-alkylguanines. Topics: Alkylation; DNA; DNA Damage; Electrophoresis, Polyacrylamide Gel; Guanine; Humans; Piperidines	1986

Article

Year

A new technique for determining the distribution of N7-methylguanine using an automated DNA sequencer.

Carcinogenesis, 1991, Volume: 12, Issue:11

We have developed a method to determine rapidly the sequence specificity of DNA alkylation resulting from chemical treatment. The utility of this approach is demonstrated here in a study of the sequence specificity of alkylation by dimethylsulphate (DMS). The method is independent of the sequence chosen and makes use of the polymerase chain reaction (PCR) to generate a fluorescently labelled DNA target. In this study, a 302 bp segment of the Escherichia coli lacI gene was amplified and the product purified by liquid chromatography on a Mono Q column. This DNA was alkylated with DMS and treated with hot piperidine to produce single-strand breaks at sites of N7 alkylation. The distribution of the break points, and hence the position and extent of alkylation, were determined on an Applied Biosystems 370A automated DNA sequencer.

Topics: Alkylation; DNA; DNA Damage; Escherichia coli; Gene Amplification; Guanine; Lac Operon; Molecular Sequence Data; Oligonucleotides; Piperidines; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Sulfuric Acid Esters

1991

Mechanism of DNA strand breakage by piperidine at sites of N7-alkylguanines.

Biochimica et biophysica acta, 1986, Oct-16, Volume: 868, Issue:1

The volatile, secondary amine piperidine is used in the Maxam-Gilbert chemical method of DNA sequencing to create strand breaks in DNA at sites of damaged bases. As such it is often used in generalized studies of DNA damage to identify 'alkali-labile lesions'. We confirm the mechanism proposed by Maxam and Gilbert (Maxam, A. and Gilbert, W. (1980) Methods Enzymol. 65, 499-560) by which aqueous piperidine creates strand breaks at sites of N7-guanine alkylations: alkaline conditions catalyze rupture of the C8-N9 bond, forming a formamido-pyrimidine structure which is displaced from the ribose moiety by piperidine. In keeping with this mechanism, the tertiary amine, N-methylpiperidine, does not catalyze the formation of strand breaks in alkylated DNA. Our data confirm the prediction that high pH in and of itself will not create strand breaks at sites of N7-alkylguanines.

Topics: Alkylation; DNA; DNA Damage; Electrophoresis, Polyacrylamide Gel; Guanine; Humans; Piperidines

1986