piperidines and (1-oxyl-2-2-5-5-tetramethylpyrroline-3-methyl)methanethiosulfonate

piperidines has been researched along with (1-oxyl-2-2-5-5-tetramethylpyrroline-3-methyl)methanethiosulfonate* in 1 studies

Reviews

1 review(s) available for piperidines and (1-oxyl-2-2-5-5-tetramethylpyrroline-3-methyl)methanethiosulfonate

Article	Year
The spin-label approach to labeling membrane protein sulfhydryl groups. Annals of the New York Academy of Sciences, 1983, Volume: 414 Labeling experiments with viable membrane preparations are optimally undertaken where conditions are extremely mild (minimal excess of modification reagent, very short reaction times) in order to reduce the possibilities of introducing artifacts (cell aging and death, denaturation, etc.). Furthermore, specificity of labeling is a critical requirement where the system is complex and contains many proteins. The spin label IV described above offers several advantages over previously employed reagents. By virtue of its reversibility and ease of quantitation, one may repetitively label and remove label from the same membrane preparation in order to check reproducibility while using the same sample as a control. Topics: Chemical Phenomena; Chemistry; Cyclic N-Oxides; Electron Spin Resonance Spectroscopy; Erythrocyte Membrane; Humans; Iodoacetamide; Iodoacetates; Maleimides; Membrane Proteins; Mesylates; Piperidines; Protein Conformation; Spin Labels; Sulfhydryl Compounds; Sulfhydryl Reagents	1983

Article

Year

The spin-label approach to labeling membrane protein sulfhydryl groups.

Annals of the New York Academy of Sciences, 1983, Volume: 414

Labeling experiments with viable membrane preparations are optimally undertaken where conditions are extremely mild (minimal excess of modification reagent, very short reaction times) in order to reduce the possibilities of introducing artifacts (cell aging and death, denaturation, etc.). Furthermore, specificity of labeling is a critical requirement where the system is complex and contains many proteins. The spin label IV described above offers several advantages over previously employed reagents. By virtue of its reversibility and ease of quantitation, one may repetitively label and remove label from the same membrane preparation in order to check reproducibility while using the same sample as a control.

Topics: Chemical Phenomena; Chemistry; Cyclic N-Oxides; Electron Spin Resonance Spectroscopy; Erythrocyte Membrane; Humans; Iodoacetamide; Iodoacetates; Maleimides; Membrane Proteins; Mesylates; Piperidines; Protein Conformation; Spin Labels; Sulfhydryl Compounds; Sulfhydryl Reagents

1983