phytosterols and stigmast-4-ene-3-6-dione

To study the chemical constituents of Abelmoschus esculentus.. The chemical constituents were isolated and purified by chromatography on silica gel and recrystallization. The chemical structures were elucidated on the basis of physicochemical properties and spectral date.. Twelve compounds were isolated and identified as 9,19-23 (Z)-cycloart-23-en-3beta, 25-diol(1), ergost-7,22-diene-3beta,5alpha,6beta-triol(2),5alpha, 6alpha-epoxyergost-8(14), 22-dien-3beta, 7alpha-diol(3), 5alpha,8alpha-epidioxyergost-22-en-3beta-ol(4), stigmast-5-ene-3beta,7alpha-diol(5), stigmast-5, 22-diene-3beta,7alpha-diol(6), stigmast-4-en-3beta,6beta-diol(7), stigmast-4,22-diene-3beta,6beta-diol(8), stigmast-4-ene-3, 6-dione(9), stigmast-4-ene-3-one (10),beta-sitosterol(11), daucosterol(12).. Compounds 1-10 are obtained from the plant for the first time and also from the genu for the first time.

Topics: Abelmoschus; Cholestenones; Chromatography, Thin Layer; Ergosterol; Fruit; Molecular Structure; Phytosterols; Solvents; Steroids; Stigmasterol

Using modern NMR techniques, including DQF-COSY, NOESY, HETCOR and HMBC, the full assignments of all 1H and 13C chemical shifts were made for stigmastane-3,6-dione and stigmast-4-ene-3,6-dione, isolated from the roots of Piper nigrum (Piperaceae). Their stereochemistry was resolved by a combination of 2D NOESY data and analysis of coupling constants. The two compounds were isolated from the genus Piper for the first time.

Topics: Chloroform; Cholestenones; Magnetic Resonance Spectroscopy; Models, Molecular; Phytosterols; Piper nigrum; Plant Roots; Solvents