phytoestrogens has been researched along with shikonin* in 1 studies
1 other study(ies) available for phytoestrogens and shikonin
| Article | Year |
|---|---|
Assessment of shikonin for potential estrogenic activity by dual-luciferase reporter based bioluminescent measurements in vitro.
Shikonin, an active component of Lithospermum erythrorhizon Sieb. et Zucc., shows multiple pharmacological properties. However, the estrogenic activity of shikonin is remaining unclear. We assessed the potential estrogenic activity of shikonin with dual-luciferase reporter assay and bioluminescent measurements, by using transient cotransfection with estrogen dependent plasmid pERE-TK-Luc and internal control plasmid pRL-TK in MCF-7 cells. Estrogenic activity of shikonin, even at high concentration did not alter significantly compared to negative control (p > 0.05) and were significantly lower than those with E2 (p < 0.01). Concluding, shikonin demonstrates no estrogenic activity in vitro. Topics: Cell Line, Tumor; Genes, Reporter; Humans; Indicators and Reagents; Luciferases; Luminescence; Medicine, Chinese Traditional; Naphthoquinones; Phytoestrogens; Plasmids; Transfection | 2011 |