phosphorus-radioisotopes and triethylamine

As a new approach to DNA adductomics, we directly reacted intact, double-stranded (ds)-DNA under warm conditions with an alkylating mass tag followed by analysis by liquid chromatography/mass spectrometry. This method is based on the tendency of adducted nucleobases to locally disrupt the DNA structure (forming a "DNA bubble") potentially increasing exposure of their nucleophilic (including active hydrogen) sites for preferential alkylation. Also encouraging this strategy is that the scope of nucleotide excision repair is very broad, and this system primarily recognizes DNA bubbles.. CAX-Prelabeling is an emerging new technique for DNA adductomics, providing polar DNA adductomics in a practical way for the first time. Further study of the method is encouraged to better characterize and extend its performance, especially in scope and sensitivity.

Topics: Animals; Benzo(a)pyrene; Benzyl Compounds; Cations; Cattle; Chromatography, High Pressure Liquid; DNA Adducts; Ethylamines; Guanine; Humans; Nucleotides; Phosphorus Radioisotopes; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Uracil